Detailed information of TA system TA006099

Experimentally validated

Overview

TA module

• Type: V
• Classification (family/domain): ghoTS/-
• Location: 4352584..4353081
• Replicon: chromosome
• Accession: NC_000913
• Organism: Escherichia coli str. K-12 substr. MG1655

Toxin (Protein)

• Gene name: ghoT
• Uniprot ID: P64646
• Locus tag: b4559
• Protein ID: YP_588474.1
• Coordinates: 4352908..4353081 (+)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: ghoS
• Uniprot ID: P0AF61
• Locus tag: b4128
• Protein ID: NP_418552.1
• Coordinates: 4352584..4352880 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
b4124	4349315..4350034	-	720	NP_418548.1	DNA-binding transcriptional activator DcuR	-
b4125	4350031..4351662	-	1632	NP_418549.1	sensor histidine kinase DcuS	-
b4126	4351843..4352073	+	231	NP_418550.1	PF06902 family protein YjdI	-
b4127	4352085..4352357	+	273	NP_418551.1	putative N-acetyltransferase YjdJ	-
b4128	4352584..4352880	+	297	NP_418552.1	antitoxin of the GhoTS toxin-antitoxin system	Antitoxin
b4559	4352908..4353081	+	174	YP_588474.1	toxin of the GhoTS toxin-antitoxin system	Toxin
b4129	4353200..4354717	-	1518	NP_418553.1	lysine--tRNA ligase/Ap4A synthetase/Ap3A synthetase	-
b4130	4354954..4356411	-	1458	NP_418554.1	dipeptide/tripeptide:H(+) symporter DtpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin GhoT/OrtT (4-57)

Antitoxin

• Endoribonuclease antitoxin GhoS (5-94)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6555.06 Da
• Isoelectric Point: 10.2288

>T6099 YP_588474.1 NC_000913:4352908-4353081 [Escherichia coli str. K-12 substr. MG1655]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Nucleotide

• Length: 174 bp

>T6099 NC_000913:4352908-4353081 [Escherichia coli str. K-12 substr. MG1655]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11467.97 Da
• Isoelectric Point: 4.1705

>AT6099 NP_418552.1 NC_000913:4352584-4352880 [Escherichia coli str. K-12 substr. MG1655]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Nucleotide

• Length: 297 bp

>AT6099 NC_000913:4352584-4352880 [Escherichia coli str. K-12 substr. MG1655]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: P64646

Antitoxin

• PDB: 2LLZ
• AlphaFold DB: P0AF61

References

(1) Xiaoxue Wang et al. (2012) A new type V toxin-antitoxin system where mRNA for toxin GhoT is cleaved by antitoxin GhoS. Nature Chemical Biology 8(10):855-61. [PubMed:22941047]

(2) Jun-Seob Kim et al. (2018) GhoT of the GhoT/GhoS toxin/antitoxin system damages lipid membranes by forming transient pores. Biochemical And Biophysical Research Communications 497(2):467-472. [PubMed:29470981]


 experimental literature