Detailed information of TA system
Experimentally validatedOverview
TA module
Type | V | Classification (family/domain) | ghoTS/- |
Location | 4352584..4353081 | Replicon | chromosome |
Accession | NC_000913 | ||
Organism | Escherichia coli str. K-12 substr. MG1655 |
Toxin (Protein)
Gene name | ghoT | Uniprot ID | P64646 |
Locus tag | b4559 | Protein ID | YP_588474.1 |
Coordinates | 4352908..4353081 (+) | Length | 58 a.a. |
Antitoxin (Protein)
Gene name | ghoS | Uniprot ID | P0AF61 |
Locus tag | b4128 | Protein ID | NP_418552.1 |
Coordinates | 4352584..4352880 (+) | Length | 99 a.a. |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
b4124 | 4349315..4350034 | - | 720 | NP_418548.1 | DNA-binding transcriptional activator DcuR | - |
b4125 | 4350031..4351662 | - | 1632 | NP_418549.1 | sensor histidine kinase DcuS | - |
b4126 | 4351843..4352073 | + | 231 | NP_418550.1 | PF06902 family protein YjdI | - |
b4127 | 4352085..4352357 | + | 273 | NP_418551.1 | putative N-acetyltransferase YjdJ | - |
b4128 | 4352584..4352880 | + | 297 | NP_418552.1 | antitoxin of the GhoTS toxin-antitoxin system | Antitoxin |
b4559 | 4352908..4353081 | + | 174 | YP_588474.1 | toxin of the GhoTS toxin-antitoxin system | Toxin |
b4129 | 4353200..4354717 | - | 1518 | NP_418553.1 | lysine--tRNA ligase/Ap4A synthetase/Ap3A synthetase | - |
b4130 | 4354954..4356411 | - | 1458 | NP_418554.1 | dipeptide/tripeptide:H(+) symporter DtpC | - |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
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Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 58 a.a. Molecular weight: 6555.06 Da Isoelectric Point: 10.2288
>T6099 YP_588474.1 NC_000913:4352908-4353081 [Escherichia coli str. K-12 substr. MG1655]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF
Download Length: 174 bp
>T6099 NC_000913:4352908-4353081 [Escherichia coli str. K-12 substr. MG1655]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG
Antitoxin
Download Length: 99 a.a. Molecular weight: 11467.97 Da Isoelectric Point: 4.1705
>AT6099 NP_418552.1 NC_000913:4352584-4352880 [Escherichia coli str. K-12 substr. MG1655]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK
Download Length: 297 bp
>AT6099 NC_000913:4352584-4352880 [Escherichia coli str. K-12 substr. MG1655]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG
Similar Proteins
Only experimentally validated proteins are listed.
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
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Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
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References
(1) Xiaoxue Wang et al. (2012) A new type V toxin-antitoxin system where mRNA for toxin GhoT is cleaved by antitoxin GhoS. Nature Chemical Biology 8(10):855-61. [PubMed:22941047]
(2) Jun-Seob Kim et al. (2018) GhoT of the GhoT/GhoS toxin/antitoxin system damages lipid membranes by forming transient pores. Biochemical And Biophysical Research Communications 497(2):467-472. [PubMed:29470981]
experimental literature