Detailed information of TA system TA060935

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2233494..2234023
• Replicon: chromosome
• Accession: NZ_CP014387
• Organism: Staphylococcus aureus strain USA300-SUR8

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: AYM21_RS11840
• Protein ID: WP_000621175.1
• Coordinates: 2233494..2233856 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: AYM21_RS11845
• Protein ID: WP_000948331.1
• Coordinates: 2233853..2234023 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYM21_RS11815	2230472..2231242	-	771	WP_001041103.1	RNA polymerase sigma factor SigB	-
AYM21_RS11820	2231217..2231696	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
AYM21_RS11825	2231698..2232024	-	327	WP_001052491.1	anti-sigma factor antagonist	-
AYM21_RS11830	2232143..2233144	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
AYM21_RS11840	2233494..2233856	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AYM21_RS11845	2233853..2234023	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
AYM21_RS11850	2234108..2235256	-	1149	WP_001281145.1	alanine racemase	-
AYM21_RS11855	2235322..2235681	-	360	WP_000581200.1	holo-ACP synthase	-
AYM21_RS11860	2235685..2236176	-	492	WP_001205910.1	PH domain-containing protein	-
AYM21_RS11865	2236163..2237746	-	1584	WP_050961224.1	PH domain-containing protein	-
AYM21_RS11870	2237739..2238218	-	480	WP_001287088.1	hypothetical protein	-
AYM21_RS11875	2238426..2238986	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T60935 WP_000621175.1 NZ_CP014387:c2233856-2233494 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T60935 NZ_CP014387:c2233856-2233494 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT60935 WP_000948331.1 NZ_CP014387:c2234023-2233853 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT60935 NZ_CP014387:c2234023-2233853 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1