Detailed information of TA system    

experimentalExperimentally validated    

Overview


TA module


Type II Classification (family/domain) phd-doc/Fic(toxin)
Location 1365336..1365922 Replicon chromosome
Accession NC_008596
Organism Mycobacterium smegmatis str. MC2 155

Toxin (Protein)


Gene name doc Uniprot ID A0A8B4QHV5
Locus tag MSMEG_RS06245 Protein ID WP_011727560.1
Coordinates 1365530..1365922 (+) Length 131 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID A0A2U9PKT9
Locus tag MSMEG_RS06240 Protein ID WP_011727559.1
Coordinates 1365336..1365533 (+) Length 66 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MSMEG_RS06215 (MSMEG_1272) 1360369..1361847 - 1479 WP_011727554.1 ADP-ribosylglycohydrolase family protein -
MSMEG_RS06220 (MSMEG_1273) 1362036..1362377 - 342 WP_011727555.1 DUF732 domain-containing protein -
MSMEG_RS06225 (MSMEG_1274) 1362437..1363564 - 1128 WP_011727556.1 SMP-30/gluconolactonase/LRE family protein -
MSMEG_RS06230 (MSMEG_1275) 1363745..1364824 - 1080 WP_011727557.1 HNH endonuclease -
MSMEG_RS06235 (MSMEG_1276) 1364892..1365188 + 297 WP_011727558.1 nucleoside triphosphate pyrophosphohydrolase -
MSMEG_RS06240 (MSMEG_1277) 1365336..1365533 + 198 WP_011727559.1 type II toxin-antitoxin system antitoxin Phd Antitoxin
MSMEG_RS06245 (MSMEG_1278) 1365530..1365922 + 393 WP_011727560.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
MSMEG_RS06250 (MSMEG_1279) 1365999..1367429 + 1431 WP_003892661.1 DUF3375 domain-containing protein -
MSMEG_RS06255 (MSMEG_1280) 1367426..1368001 + 576 Protein_1244 DUF4194 domain-containing protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(49-110)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 131 a.a.        Molecular weight: 13939.57 Da        Isoelectric Point: 4.2481

>T6093 WP_011727560.1 NC_008596:1365530-1365922 [Mycolicibacterium smegmatis MC2 155]
VTEYLDREDVLTAGSIAFGGELKVRDYGLLDAAVARPQATVYGVDAYPRLWDKAAALLQSLARNHALVDGNKRTAWAAAW
TFLHINGVQLAADFDVDRAEDLMNEVATRDCDLDSIAAELAGFAAAAQTG

Download         Length: 393 bp

>T6093 NC_008596:1365530-1365922 [Mycolicibacterium smegmatis MC2 155]
GTGACCGAGTACCTCGACCGCGAGGACGTGCTCACCGCGGGATCGATCGCTTTTGGCGGCGAGCTGAAGGTGCGGGACTA
CGGACTGCTCGACGCTGCGGTTGCCCGCCCGCAGGCCACGGTGTATGGCGTGGATGCGTACCCGCGGCTGTGGGACAAGG
CCGCTGCGTTGTTGCAGTCCCTGGCGCGGAACCATGCGCTGGTCGACGGTAACAAGCGCACTGCGTGGGCTGCGGCATGG
ACATTTCTGCACATCAACGGTGTACAGCTGGCGGCTGATTTCGATGTCGATCGCGCGGAGGACCTCATGAACGAGGTGGC
CACCCGTGACTGCGACCTGGATTCCATCGCCGCCGAGCTGGCCGGTTTCGCCGCTGCTGCGCAGACGGGGTGA

Antitoxin


Download         Length: 66 a.a.        Molecular weight: 7276.30 Da        Isoelectric Point: 8.9353

>AT6093 WP_011727559.1 NC_008596:1365336-1365533 [Mycolicibacterium smegmatis MC2 155]
MPSLNIDFDEAEMEQIRAAARADDLSLKKFAHAAVMERASAHKRRVAEAARLVAERSAELNRRLA

Download         Length: 198 bp

>AT6093 NC_008596:1365336-1365533 [Mycolicibacterium smegmatis MC2 155]
ATGCCGTCGCTGAACATCGACTTCGACGAAGCCGAGATGGAACAGATCCGCGCTGCTGCCCGGGCGGATGACCTGTCGCT
GAAGAAGTTCGCGCACGCCGCCGTGATGGAGCGTGCCAGCGCGCACAAGCGCAGGGTCGCCGAGGCGGCGCGTCTGGTCG
CTGAGCGTTCGGCTGAGCTCAACCGCCGCTTGGCGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A8B4QHV5


Antitoxin

Source ID Structure
AlphaFold DB A0A2U9PKT9

References


(1) Rebekah Frampton et al. (2012) Toxin-antitoxin systems of Mycobacterium smegmatis are essential for cell survival. The Journal of Biological Chemistry 287(8):5340-56. [PubMed:22199354] experimental


experimental experimental literature