60800

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 262220..262806 Replicon plasmid unnamed
Accession NZ_CP014361
Organism Methylomonas sp. DH-1

Toxin (Protein)


Gene name mazF Uniprot ID A0A172UFF8
Locus tag AYM39_RS22660 Protein ID WP_064023820.1
Coordinates 262220..262558 (-) Length 113 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A172UGM9
Locus tag AYM39_RS22665 Protein ID WP_064023821.1
Coordinates 262558..262806 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AYM39_RS24210 258002..258211 + 210 WP_157198358.1 hypothetical protein -
AYM39_RS22635 258182..258472 - 291 WP_157198359.1 hypothetical protein -
AYM39_RS22640 258583..258819 + 237 WP_054758403.1 hypothetical protein -
AYM39_RS24215 258834..259064 - 231 WP_054758400.1 hypothetical protein -
AYM39_RS22645 259374..260372 - 999 WP_064023818.1 YqaJ viral recombinase family protein -
AYM39_RS22650 260369..260716 - 348 WP_082881142.1 hypothetical protein -
AYM39_RS22655 260807..261772 - 966 WP_157198365.1 phage recombination protein Bet -
AYM39_RS22660 262220..262558 - 339 WP_064023820.1 endoribonuclease MazF Toxin
AYM39_RS22665 262558..262806 - 249 WP_064023821.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
AYM39_RS22675 263126..263353 - 228 WP_064023823.1 hypothetical protein -
AYM39_RS22680 263686..264591 + 906 WP_064023824.1 DNA-binding protein -
AYM39_RS22685 265418..265873 + 456 WP_064023825.1 RHS repeat protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - cyaB 1..277875 277875


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-110)

Antitoxin

SpoVT-AbrB domain

(6-46)


Sequences

Toxin        


Download         Length: 113 a.a.        Molecular weight: 12110.03 Da        Isoelectric Point: 5.8269

>T60800 WP_064023820.1 NZ_CP014361:c262558-262220 [Methylomonas sp. DH-1]
MAVSYVPERGDLVWIAFDPQAGHEQAGRRPGLVVTPRTYNVKAGLALICPVTSRVKGYPFEVPLPDGDRVSGVILADQIR
SLDWAARQAEKISMVDGAVLAEVLAKIEALIQ

Download         Length: 339 bp

>T60800 NZ_CP014361:c262558-262220 [Methylomonas sp. DH-1]
ATGGCTGTATCGTACGTACCTGAGCGCGGGGATTTGGTGTGGATTGCGTTCGATCCTCAAGCAGGCCACGAACAAGCGGG
GCGCCGCCCCGGCCTGGTCGTTACGCCGCGCACTTATAACGTCAAAGCCGGGTTGGCGTTGATTTGCCCTGTAACAAGTC
GGGTTAAAGGATATCCGTTTGAGGTACCGTTACCGGACGGCGACCGTGTGAGTGGTGTGATATTGGCGGATCAGATTCGG
AGTCTGGATTGGGCGGCGCGTCAGGCCGAAAAGATATCTATGGTGGACGGGGCGGTATTGGCCGAAGTCTTGGCCAAAAT
CGAGGCTCTTATTCAATAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8902.15 Da        Isoelectric Point: 5.0196

>AT60800 WP_064023821.1 NZ_CP014361:c262806-262558 [Methylomonas sp. DH-1]
MHTEIKKWGNSAVVRLPAAMLAQLKLEIGSPVELTADDGGFRIVPSTAKPVFKLDELLAGMTSDNRHDCIDWGPDVGREG
NN

Download         Length: 249 bp

>AT60800 NZ_CP014361:c262806-262558 [Methylomonas sp. DH-1]
ATGCACACCGAAATTAAAAAATGGGGTAATAGCGCCGTGGTTCGATTGCCGGCGGCGATGCTTGCACAGCTAAAGCTGGA
AATCGGTTCGCCGGTCGAACTGACGGCTGATGATGGCGGGTTTCGTATTGTGCCGTCCACGGCTAAGCCTGTTTTCAAGT
TGGACGAGTTGCTGGCTGGCATGACTTCCGATAATCGGCATGATTGCATTGACTGGGGGCCGGATGTTGGCCGAGAAGGT
AACAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A172UFF8

Antitoxin

Source ID Structure
AlphaFold DB A0A172UGM9

References