Detailed information of TA system TA060799

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 237274..237845
• Replicon: plasmid unnamed
• Accession: NZ_CP014361
• Organism: Methylomonas sp. DH-1

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: AYM39_RS22510
• Protein ID: WP_064023796.1
• Coordinates: 237274..237564 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A172UFL5
• Locus tag: AYM39_RS22515
• Protein ID: WP_064023797.1
• Coordinates: 237552..237845 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYM39_RS23475	232447..232716	+	270	WP_157198353.1	multiubiquitin domain-containing protein	-
AYM39_RS22490	232691..233881	+	1191	WP_064023794.1	ThiF family adenylyltransferase	-
AYM39_RS22495	234058..235305	-	1248	WP_064023669.1	IS256 family transposase	-
AYM39_RS22500	235465..236109	-	645	Protein_201	SOS response-associated peptidase	-
AYM39_RS22505	236306..237274	-	969	WP_064023795.1	tyrosine-type recombinase/integrase	-
AYM39_RS22510	237274..237564	-	291	WP_064023796.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AYM39_RS22515	237552..237845	-	294	WP_064023797.1	ribbon-helix-helix protein, CopG family	Antitoxin
AYM39_RS22520	238046..238240	+	195	WP_064023798.1	hypothetical protein	-
AYM39_RS22525	238503..238739	+	237	WP_064023799.1	ribbon-helix-helix protein, CopG family	-
AYM39_RS22530	238726..239079	+	354	WP_064023800.1	type II toxin-antitoxin system VapC family toxin	-
AYM39_RS22535	239090..240564	+	1475	Protein_208	SAM-dependent DNA methyltransferase	-
AYM39_RS23480	240865..241152	+	288	WP_082881152.1	hypothetical protein	-
AYM39_RS22540	241162..241962	+	801	WP_064023801.1	retroviral-like aspartic protease family protein	-
AYM39_RS22545	242208..242459	+	252	WP_064023802.1	type II toxin-antitoxin system ParD family antitoxin	-
AYM39_RS22550	242456..242776	+	321	WP_064023803.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	cyaB	1..277875	277875
	-	flank	IS/Tn	-	-	234058..235305	1247

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-93)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-50)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10764.38 Da
• Isoelectric Point: 10.5386

>T60799 WP_064023796.1 NZ_CP014361:c237564-237274 [Methylomonas sp. DH-1]
VSQIVWLPDALMDVERLRAFLQEKNPKAAARAGQTLRDGASALAGFPQIGRPMNDGSERRELFLPFGAGAYVLRYRINGD
TVVIIRVWHSKEKREA

Nucleotide

• Length: 291 bp

>T60799 NZ_CP014361:c237564-237274 [Methylomonas sp. DH-1]
GTGTCCCAAATAGTCTGGCTTCCCGACGCCTTGATGGATGTCGAGCGCTTGCGGGCATTCCTTCAAGAAAAGAATCCAAA
GGCTGCCGCGCGCGCCGGGCAAACGTTACGCGATGGGGCCAGCGCTCTTGCTGGCTTCCCCCAGATTGGACGCCCCATGA
ATGATGGATCGGAGCGTCGAGAACTCTTTTTGCCGTTCGGCGCCGGCGCCTATGTTCTGCGTTACCGGATTAATGGCGAT
ACTGTCGTCATTATTCGGGTTTGGCATAGCAAGGAAAAGCGGGAGGCTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11364.76 Da
• Isoelectric Point: 5.6868

>AT60799 WP_064023797.1 NZ_CP014361:c237845-237552 [Methylomonas sp. DH-1]
MKQTDTLSKTISTGIKLDETLHARLKLLSQAKDRTPHWLMKAAIEEYVEREEAYEREKREDMERWQKYQQTGHAIPNEAV
DAWLSSWGSESELSCPK

Nucleotide

• Length: 294 bp

>AT60799 NZ_CP014361:c237845-237552 [Methylomonas sp. DH-1]
ATGAAACAAACTGACACACTATCAAAGACCATCAGCACCGGCATCAAGCTGGACGAGACTCTTCATGCTCGACTGAAGCT
ACTGAGCCAGGCCAAAGACCGAACGCCTCACTGGCTGATGAAGGCCGCCATCGAGGAATATGTCGAACGCGAAGAAGCGT
ATGAGCGTGAAAAGCGCGAAGACATGGAGCGTTGGCAGAAATACCAACAAACCGGCCATGCCATACCAAATGAAGCCGTC
GATGCCTGGCTCTCATCCTGGGGAAGCGAAAGTGAGCTATCGTGTCCCAAATAG

Structures

Antitoxin

• AlphaFold DB: A0A172UFL5