Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN-MazE
Location 51324..51960 Replicon plasmid unnamed
Accession NZ_CP014361
Organism Methylomonas sp. DH-1

Toxin (Protein)


Gene name vapC Uniprot ID A0A172UG52
Locus tag AYM39_RS21780 Protein ID WP_064023682.1
Coordinates 51324..51740 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A172UFQ6
Locus tag AYM39_RS21785 Protein ID WP_064023836.1
Coordinates 51727..51960 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AYM39_RS21750 46787..47185 + 399 WP_157198335.1 hypothetical protein -
AYM39_RS21755 47188..47820 + 633 WP_054759906.1 DUF4400 domain-containing protein -
AYM39_RS21760 47835..48326 + 492 WP_064023679.1 thermonuclease family protein -
AYM39_RS21765 48323..48691 + 369 WP_054759910.1 hypothetical protein -
AYM39_RS21770 48708..50120 + 1413 WP_064023680.1 MBOAT family protein -
AYM39_RS21775 50092..51129 + 1038 WP_157198336.1 hypothetical protein -
AYM39_RS21780 51324..51740 - 417 WP_064023682.1 PIN domain-containing protein Toxin
AYM39_RS21785 51727..51960 - 234 WP_064023836.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
AYM39_RS23405 52209..52406 + 198 WP_082881126.1 cold shock domain-containing protein -
AYM39_RS21795 52542..54548 - 2007 WP_064023684.1 exodeoxyribonuclease V subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - cyaB 1..277875 277875


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-116)

Antitoxin

SpoVT-AbrB domain

(5-44)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15098.36 Da        Isoelectric Point: 4.4790

>T60798 WP_064023682.1 NZ_CP014361:c51740-51324 [Methylomonas sp. DH-1]
MKTAKPFLDTNVLLYLLSEDSQKADRAEEIIAFGGIINVQVLNEFASVASRKLKMTYVEIRETLSIIRSICQTQALSVSI
HEQGLAIAEQFGFSLYDSMIVSAALDAGCSILYSEDMQHGQAIGSLVINNPFLPSLPN

Download         Length: 417 bp

>T60798 NZ_CP014361:c51740-51324 [Methylomonas sp. DH-1]
ATGAAAACCGCTAAGCCGTTTCTCGATACCAACGTCTTGCTGTATTTACTGTCTGAGGACAGTCAAAAAGCAGACCGCGC
CGAAGAGATTATCGCCTTCGGCGGCATAATCAATGTGCAGGTGCTGAATGAATTTGCGTCGGTAGCGTCGCGCAAACTGA
AGATGACGTATGTCGAAATCCGCGAAACCTTGTCGATTATCCGAAGTATCTGTCAAACTCAAGCTTTGAGCGTCAGCATC
CACGAACAAGGTTTGGCGATTGCCGAACAATTTGGTTTTTCGTTGTATGACAGCATGATTGTCAGCGCGGCGCTGGACGC
CGGGTGCTCAATATTGTATTCGGAAGACATGCAACATGGTCAAGCTATTGGCTCGTTAGTCATTAACAATCCGTTTTTAC
CTTCTCTTCCTAACTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8756.09 Da        Isoelectric Point: 7.2054

>AT60798 WP_064023836.1 NZ_CP014361:c51960-51727 [Methylomonas sp. DH-1]
MQVAKWGNSLAIRLPATVVEALELKEGDDIEVHIAGERVFELEKKPSAKTLLAKLHQFRGRLPADFKFDRLEANENR

Download         Length: 234 bp

>AT60798 NZ_CP014361:c51960-51727 [Methylomonas sp. DH-1]
ATGCAAGTCGCAAAATGGGGTAATAGTTTAGCTATTCGTTTGCCCGCCACGGTGGTAGAAGCGTTAGAGTTAAAGGAAGG
CGATGACATTGAAGTACATATCGCCGGCGAACGGGTGTTTGAACTAGAGAAAAAACCTTCGGCAAAGACGTTGTTAGCCA
AATTGCATCAATTTCGTGGGCGCCTACCCGCTGATTTTAAGTTTGACCGCCTGGAAGCGAATGAAAACCGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A172UG52

Antitoxin

Source ID Structure
AlphaFold DB A0A172UFQ6

References