Detailed information of TA system TA060794

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 3474416..3474951
• Replicon: chromosome
• Accession: NZ_CP014360
• Organism: Methylomonas sp. DH-1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A172UAT0
• Locus tag: AYM39_RS15390
• Protein ID: WP_064022364.1
• Coordinates: 3474628..3474951 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: AYM39_RS15385
• Protein ID: WP_197495868.1
• Coordinates: 3474416..3474631 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYM39_RS15365	3470488..3471585	+	1098	WP_064019980.1	IS5 family transposase	-
AYM39_RS15370	3471763..3472389	+	627	WP_064022362.1	hypothetical protein	-
AYM39_RS15375	3472373..3473368	+	996	WP_064022363.1	DUF262 domain-containing protein	-
AYM39_RS15380	3473619..3474162	+	544	Protein_3109	transposase	-
AYM39_RS15385	3474416..3474631	+	216	WP_197495868.1	antitoxin MazE family protein	Antitoxin
AYM39_RS15390	3474628..3474951	+	324	WP_064022364.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AYM39_RS15395	3475344..3476321	+	978	WP_064022365.1	IS110 family transposase	-
AYM39_RS15400	3476570..3479902	-	3333	WP_064022366.1	NAD-glutamate dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-103)

Antitoxin

• Antitoxin MazE-like (8-70)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11419.26 Da
• Isoelectric Point: 7.3201

>T60794 WP_064022364.1 NZ_CP014360:3474628-3474951 [Methylomonas sp. DH-1]
MRRGDLVTVALQGDLGKPRPALLIQSDLFDAHPSVTILPVTGELRDAPLFRIPLAANEHTGLSKPSQVMVDKPQSVSRSK
IGAVIGHVDDETLLAVNRALAVFLGFA

Nucleotide

• Length: 324 bp

>T60794 NZ_CP014360:3474628-3474951 [Methylomonas sp. DH-1]
ATGAGGCGCGGCGATCTAGTGACGGTCGCGTTACAGGGCGATCTTGGCAAACCCAGACCGGCGCTGCTGATTCAGTCCGA
CTTGTTCGACGCGCACCCATCCGTGACGATTTTGCCGGTTACCGGCGAGTTACGCGATGCACCGTTGTTTCGAATCCCCT
TGGCGGCCAATGAACATACCGGGCTTAGCAAGCCTTCGCAAGTGATGGTGGATAAGCCGCAATCCGTGTCGCGCAGTAAA
ATCGGCGCCGTTATCGGACATGTGGATGACGAAACCCTGTTGGCGGTCAACCGAGCATTGGCGGTATTTTTGGGGTTTGC
CTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8254.28 Da
• Isoelectric Point: 6.4478

>AT60794 WP_197495868.1 NZ_CP014360:3474416-3474631 [Methylomonas sp. DH-1]
MMSISTSARVQKHRQSLREAGLRPLQIWVPDTRRNGFADECRRQSLLIRDDAADQETCMWLEAVADHDGWQ

Nucleotide

• Length: 216 bp

>AT60794 NZ_CP014360:3474416-3474631 [Methylomonas sp. DH-1]
ATGATGAGTATCAGTACTTCGGCACGTGTGCAAAAACATCGCCAGTCATTAAGGGAGGCGGGGTTGCGCCCCCTGCAAAT
TTGGGTGCCCGATACCCGGCGTAATGGTTTTGCCGACGAATGCCGGCGGCAATCCTTGCTGATTCGTGATGATGCCGCGG
ATCAGGAGACTTGTATGTGGCTGGAAGCGGTGGCTGACCACGACGGCTGGCAATGA

Structures

Toxin

• AlphaFold DB: A0A172UAT0