Detailed information of TA system TA060789

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-StbC
• Location: 881219..881861
• Replicon: chromosome
• Accession: NZ_CP014360
• Organism: Methylomonas sp. DH-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A172U5G4
• Locus tag: AYM39_RS04055
• Protein ID: WP_064020613.1
• Coordinates: 881442..881861 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: AYM39_RS22835
• Protein ID: WP_082880795.1
• Coordinates: 881219..881455 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYM39_RS04025	876287..876511	-	225	WP_064020607.1	hypothetical protein	-
AYM39_RS04030	876548..877072	-	525	WP_157198063.1	hypothetical protein	-
AYM39_RS04035	877163..877459	-	297	WP_064020609.1	HigA family addiction module antidote protein	-
AYM39_RS04040	877482..878489	-	1008	WP_064020610.1	DUF4325 domain-containing protein	-
AYM39_RS04045	878632..879834	-	1203	WP_064020611.1	site-specific integrase	-
AYM39_RS04050	880020..880811	-	792	WP_064020612.1	hypothetical protein	-
AYM39_RS22835	881219..881455	+	237	WP_082880795.1	DNA-binding protein	Antitoxin
AYM39_RS04055	881442..881861	+	420	WP_064020613.1	type II toxin-antitoxin system VapC family toxin	Toxin
AYM39_RS04060	881955..882968	-	1014	WP_064020614.1	hypothetical protein	-
AYM39_RS04065	882965..883540	-	576	WP_157198064.1	hypothetical protein	-
AYM39_RS04070	883635..884861	+	1227	WP_064020593.1	IS256 family transposase	-
AYM39_RS04075	885011..886258	-	1248	WP_064023362.1	IS256 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15786.13 Da
• Isoelectric Point: 5.1661

>T60789 WP_064020613.1 NZ_CP014360:881442-881861 [Methylomonas sp. DH-1]
MYLIDTNVISEIRKGDKANPGVRRFFDAAIQNNQKLYISVVTIGELRRGVDLIFHRGDISQSKLLETWLNSILEQYQENI
LGIDCDIALIWGKMRVPNPQHPIDKLIAATALIYDLTVVTRNTEDFQNTGIALLNPFSE

Nucleotide

• Length: 420 bp

>T60789 NZ_CP014360:881442-881861 [Methylomonas sp. DH-1]
ATGTATTTGATTGATACTAACGTCATCAGTGAGATCAGAAAAGGCGACAAAGCCAATCCAGGGGTTCGCCGTTTTTTTGA
TGCTGCAATCCAAAATAATCAAAAACTTTATATTTCTGTGGTCACGATTGGGGAGCTACGCAGAGGTGTCGATTTGATTT
TTCATCGCGGTGACATCTCGCAAAGCAAGCTTTTAGAAACGTGGCTAAATTCGATTCTTGAACAATATCAAGAGAACATT
CTCGGTATTGACTGCGATATAGCTTTAATCTGGGGGAAAATGCGCGTTCCTAATCCACAGCATCCCATCGACAAGCTGAT
TGCGGCAACTGCATTGATTTATGATTTGACGGTTGTGACTCGTAATACAGAGGATTTCCAAAACACCGGCATCGCTCTAC
TGAATCCATTTTCTGAATAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8674.77 Da
• Isoelectric Point: 5.5500

>AT60789 WP_082880795.1 NZ_CP014360:881219-881455 [Methylomonas sp. DH-1]
MPSLVVRNLDESIINALKARAVAHHRSTEAEHRAILAEFLLSPQRKTFAEALTNMPSVGIDTDFQRVNDDTPATNVFD

Nucleotide

• Length: 237 bp

>AT60789 NZ_CP014360:881219-881455 [Methylomonas sp. DH-1]
ATGCCTAGTCTAGTTGTCAGAAACCTTGATGAATCCATCATTAACGCGCTTAAAGCGAGAGCAGTTGCACATCACCGCTC
TACCGAAGCGGAGCACCGCGCGATTTTAGCGGAGTTTTTGTTAAGTCCCCAACGTAAAACCTTTGCAGAGGCATTAACCA
ATATGCCTTCTGTCGGTATAGATACCGATTTTCAACGCGTGAATGACGACACTCCGGCAACCAATGTATTTGATTGA

Structures

Toxin

• AlphaFold DB: A0A172U5G4