Detailed information of TA system TA060781

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 3824187..3824825
• Replicon: chromosome
• Accession: NZ_CP014348
• Organism: Escherichia coli str. K-12 substr. MG1655 strain JW5437-1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: AWN69_RS18865
• Protein ID: WP_000813794.1
• Coordinates: 3824649..3824825 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: AWN69_RS18860
• Protein ID: WP_001270286.1
• Coordinates: 3824187..3824603 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AWN69_RS18840	3819339..3820280	-	942	WP_001251304.1	ABC transporter permease	-
AWN69_RS18845	3820281..3821294	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
AWN69_RS18850	3821312..3822457	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
AWN69_RS18855	3822702..3824108	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
AWN69_RS18860	3824187..3824603	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
AWN69_RS18865	3824649..3824825	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
AWN69_RS18870	3825047..3825277	+	231	WP_000494244.1	YncJ family protein	-
AWN69_RS18875	3825369..3827330	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
AWN69_RS18880	3827403..3827939	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
AWN69_RS18885	3827992..3829206	+	1215	WP_001326689.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T60781 WP_000813794.1 NZ_CP014348:c3824825-3824649 [Escherichia coli str. K-12 substr. MG1655]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T60781 NZ_CP014348:c3824825-3824649 [Escherichia coli str. K-12 substr. MG1655]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT60781 WP_001270286.1 NZ_CP014348:c3824603-3824187 [Escherichia coli str. K-12 substr. MG1655]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT60781 NZ_CP014348:c3824603-3824187 [Escherichia coli str. K-12 substr. MG1655]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB