Detailed information of TA system TA006078

Experimentally validated

Overview

TA module

• Type: II
• Classification (family/domain): lsoAB/-
• Location: 971..2388
• Replicon: plasmid pOSAK1
• Accession: NC_002127
• Organism: Escherichia coli O157:H7 str. Sakai

Toxin (Protein)

• Gene name: lsoA
• Uniprot ID: O82881
• Locus tag: pOSAK1_03
• Protein ID: NP_052606.1
• Coordinates: 1348..2388 (-)
• Length: 347 a.a.

Antitoxin (Protein)

• Gene name: lsoB
• Uniprot ID: Q7DKW4
• Locus tag: pOSAK1_02
• Protein ID: NP_052605.1
• Coordinates: 971..1351 (-)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
pOSAK1_01	413..736	+	324	NP_052604.1	plasmid mobilization	-
pOSAK1_02	971..1351	-	381	NP_052605.1	hypothetical protein	Antitoxin
pOSAK1_03	1348..2388	-	1041	NP_052606.1	hypothetical protein	Toxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NZ_CP006633 Escherichia coli PCN033 1..3319 0.007 View NZ_CP012804 Escherichia coli O157:H7 strain WS4202 isolate laboratory strain 1..3306 0 View NZ_CP028613 Escherichia coli strain 142 1..3306 0 View NZ_CP032083 Escherichia coli strain CT30 1..3319 0.007 View NZ_CP032800 Escherichia coli strain ERL06-2497 1..3306 0 View NZ_CP032807 Escherichia coli strain ERL05-0623 1..3306 0 View NZ_CP034388 Escherichia coli O157:H7 strain FRIK804 1..3305 0 View NZ_CP038315 Escherichia coli O157:H7 strain OK1 1..3306 0 View NZ_CP038358 Escherichia coli O157:H7 strain F7508 1..3306 0 View NZ_CP038365 Escherichia coli O157:H7 strain F7349 1..3306 0 View NZ_CP038407 Escherichia coli O157:H7 strain ATCC 35150 1..3306 0 View NZ_CP038411 Escherichia coli O157:H7 strain 86-24 1..3306 0 View NZ_CP038430 Escherichia coli O157:H7 strain 611 1..3306 0 View NZ_CP039836 Escherichia coli O157:H7 strain MB41-1 1..3306 0 View NZ_CP045865 Escherichia coli O157:H7 strain ATCC 43890 1..3306 0 View NZ_CP062735 Escherichia coli O157:H7 strain Z1767 1..3306 0 View NZ_CP062738 Escherichia coli O157:H7 strain Z1766 1..3306 0 View NZ_CP062741 Escherichia coli O157:H7 strain Z1723 1..3306 0 View NZ_CP062748 Escherichia coli O157:H7 strain Z1504 1..3306 0 View NZ_CP062751 Escherichia coli O157:H7 strain Z1486 1..3306 0 View NZ_CP062754 Escherichia coli O157:H7 strain Z910 1..3306 0 View NZ_CP062757 Escherichia coli O157:H7 strain Z903 1..3306 0 View NZ_CP062760 Escherichia coli O157:H7 strain Z892 1..3306 0 View NZ_CP062765 Escherichia coli O157:H7 strain Z885 1..3306 0 View NZ_CP062768 Escherichia coli O157:H7 strain Z866 1..3306 0 View NZ_CP062773 Escherichia coli O157:H7 strain Z570 1..3306 0 View NZ_CP062777 Escherichia coli O157:H7 strain Z563 1..3306 0 View NZ_CP062786 Escherichia coli O157:H7 strain Z869 1..3306 0 View NZ_CP064168 Escherichia coli O157:H7 strain Wll001 1..3306 0.001 View NZ_CP066758 Escherichia coli O157:H7 strain ECP19-798 1..3306 0 View NZ_CP066761 Escherichia coli O157:H7 strain ECP19-198 1..3306 0 View NZ_CP070119 Escherichia coli strain FDAARGOS_1298 1..3319 0.008 View NZ_CP076240 Escherichia coli O157:H7 strain 146N4 1..3306 0 View NZ_CP076247 Escherichia coli O157:H7 strain Balcarce_24.2 1..3306 0 View NZ_CP120946 Escherichia coli O157:H7 str. EDL933 1..3306 0	inside	Mobilizable plasmid	-	-	1..3306	3306

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain (90-174)

Antitoxin

• Antitoxin RnlB/LsoB (20-114)

Sequences

Toxin

Protein

• Length: 347 a.a.
• Molecular weight: 39573.97 Da
• Isoelectric Point: 5.6793

>T6078 NP_052606.1 NC_002127:c2388-1348 [Escherichia coli O157:H7 str. Sakai]
MAQNPFKALNINIDKIESALTQNGVTNYSSNVKNERETHISGTYKGIDFLIKLMPSGGNTTIGRASGQNNTYFDEIALII
KENCLYSDTKNFEYTIPKFSDDDRANLFEFLSEEGITITEDNNNDPNCKHQYIMTTSNGDRVRAKIYKRGSIQFQGKYLQ
IASLINDFMCSILNMKEIVEQKNKEFNVDIKKETIESELHSKLPKSIDKIHEDIKKQLSCSLIMKKIDVEMEDYSTYCFS
ALRAIEGFIYQILNDVCNPSSSKNLGEYFTENKPKYIIREIHQETINGEIAEVLCECYTYWHENRHGLFHMKPGIADTKT
INKLESIAIIDTVCQLIDGGVARLKL

Nucleotide

• Length: 1041 bp

>T6078 NC_002127:c2388-1348 [Escherichia coli O157:H7 str. Sakai]
ATGGCACAGAACCCGTTTAAAGCACTAAATATCAATATTGACAAGATTGAGTCTGCTCTGACGCAGAATGGCGTCACAAA
CTATTCCTCTAATGTAAAAAACGAAAGAGAAACTCACATATCTGGCACATATAAAGGAATAGACTTCTTAATAAAACTAA
TGCCATCAGGCGGAAATACCACTATCGGAAGAGCGTCTGGGCAAAATAACACTTACTTTGATGAAATCGCCTTGATTATA
AAAGAAAACTGTTTGTATTCAGACACAAAGAACTTTGAATACACCATTCCAAAATTCAGTGATGATGACAGGGCAAATCT
ATTTGAATTTCTTTCTGAAGAGGGGATAACAATAACAGAAGATAATAATAACGATCCTAATTGTAAACACCAATATATTA
TGACCACCAGCAATGGTGACAGGGTCAGGGCAAAAATTTACAAGCGCGGCTCTATTCAATTCCAAGGAAAATACCTTCAA
ATCGCGAGTTTGATTAACGATTTCATGTGCTCAATACTAAACATGAAAGAGATTGTCGAACAAAAAAATAAAGAATTTAA
TGTTGACATAAAAAAAGAAACTATTGAGTCCGAGTTGCATAGCAAACTACCAAAAAGCATCGATAAAATCCATGAAGATA
TCAAAAAACAGCTATCATGCTCGCTAATAATGAAAAAAATAGATGTCGAAATGGAAGATTACTCAACATACTGCTTCTCT
GCATTAAGAGCCATAGAAGGCTTTATATATCAAATACTTAATGATGTTTGCAATCCATCATCATCAAAGAACCTTGGCGA
ATACTTCACTGAAAACAAACCCAAATATATAATCAGAGAAATACACCAAGAAACTATAAATGGTGAAATAGCGGAAGTTT
TGTGTGAATGCTACACTTACTGGCATGAGAACAGGCATGGTTTGTTTCATATGAAACCAGGAATAGCTGACACGAAGACA
ATTAACAAATTAGAATCAATCGCAATCATCGATACCGTTTGCCAATTAATAGATGGTGGCGTAGCTAGGTTGAAATTATG
A

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 14526.74 Da
• Isoelectric Point: 10.1423

>AT6078 NP_052605.1 NC_002127:c1351-971 [Escherichia coli O157:H7 str. Sakai]
MKKDKKYQIEAIKNKDKTLFIVYATDIYSPSEFFSKIESDLKKKKSKGDVFFDLIIPNGGKKDRYVYTSFNGEKFSSYTL
NKVTKTDEYNDLSELSASFFKKNFDKINVNLLSKATSFALKKGIPI

Nucleotide

• Length: 381 bp

>AT6078 NC_002127:c1351-971 [Escherichia coli O157:H7 str. Sakai]
ATGAAAAAAGATAAAAAATATCAAATAGAAGCAATAAAAAATAAAGATAAAACTTTATTTATTGTCTATGCTACTGATAT
TTATAGCCCGAGCGAATTTTTCTCAAAAATCGAATCCGACTTGAAGAAAAAGAAAAGCAAGGGTGATGTTTTTTTTGATT
TAATAATTCCTAACGGTGGAAAAAAAGATCGTTACGTCTATACGTCATTTAATGGCGAGAAGTTTTCAAGTTACACATTA
AACAAAGTTACGAAAACTGATGAATATAATGATTTATCTGAGCTCTCGGCTTCGTTCTTTAAAAAAAACTTTGATAAGAT
CAACGTAAACCTTCTATCCAAAGCCACATCATTTGCTTTGAAAAAAGGCATTCCAATATAA

Structures

Toxin

• PDB: 5HY3

Antitoxin

• AlphaFold DB: Q7DKW4

References

(1) Yuichi Otsuka et al. (2012) Dmd of bacteriophage T4 functions as an antitoxin against Escherichia coli LsoA and RnlA toxins. Molecular Microbiology 83(4):669-81. [PubMed:22403819]

(2) Hua Wan et al. (2016) Structural insights into the inhibition mechanism of bacterial toxin LsoA by bacteriophage antitoxin Dmd. Molecular Microbiology 101(5):757-69. [PubMed:27169810]


 experimental literature
 protein structure literature