Detailed information of TA system TA060752

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 435397..436076
• Replicon: chromosome
• Accession: NZ_CP014348
• Organism: Escherichia coli str. K-12 substr. MG1655 strain JW5437-1

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: AWN69_RS02220
• Protein ID: WP_000854672.1
• Coordinates: 435735..436076 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: AWN69_RS02215
• Protein ID: WP_000070395.1
• Coordinates: 435397..435714 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AWN69_RS02175	430444..431307	+	864	WP_001065553.1	GTPase family protein	-
AWN69_RS02180	431399..432220	+	822	WP_000197389.1	DUF945 domain-containing protein	-
AWN69_RS25615	432437..432628	+	192	Protein_428	DeoR family transcriptional regulator	-
AWN69_RS25620	432624..432851	+	228	WP_001548158.1	hypothetical protein	-
AWN69_RS25625	432851..433294	+	444	WP_000824223.1	hypothetical protein	-
AWN69_RS02195	433317..433784	+	468	WP_001547765.1	hypothetical protein	-
AWN69_RS02200	433861..434100	+	240	WP_000194654.1	DUF905 domain-containing protein	-
AWN69_RS02205	434198..434656	+	459	WP_000211838.1	antirestriction protein	-
AWN69_RS02210	434672..435148	+	477	WP_000811693.1	RadC family protein	-
AWN69_RS23395	435157..435378	+	222	WP_000691994.1	DUF987 domain-containing protein	-
AWN69_RS02215	435397..435714	+	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
AWN69_RS02220	435735..436076	+	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
AWN69_RS02230	436648..437901	-	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
AWN69_RS02235	437913..439016	-	1104	WP_001285288.1	glutamate 5-kinase	-
AWN69_RS02240	439304..440359	+	1056	WP_000749863.1	phosphoporin PhoE	-
AWN69_RS02245	440398..440799	-	402	WP_000174689.1	sigma factor-binding protein Crl	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T60752 WP_000854672.1 NZ_CP014348:435735-436076 [Escherichia coli str. K-12 substr. MG1655]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T60752 NZ_CP014348:435735-436076 [Escherichia coli str. K-12 substr. MG1655]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT60752 WP_000070395.1 NZ_CP014348:435397-435714 [Escherichia coli str. K-12 substr. MG1655]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT60752 NZ_CP014348:435397-435714 [Escherichia coli str. K-12 substr. MG1655]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684