Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 218585..219203 Replicon chromosome
Accession NZ_CP014348
Organism Escherichia coli str. K-12 substr. MG1655 strain JW5437-1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AWN69_RS01095 Protein ID WP_001291435.1
Coordinates 218985..219203 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AWN69_RS01090 Protein ID WP_000344800.1
Coordinates 218585..218959 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AWN69_RS01080 213674..214867 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
AWN69_RS01085 214890..218039 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
AWN69_RS01090 218585..218959 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AWN69_RS01095 218985..219203 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
AWN69_RS01100 219375..219926 + 552 WP_000102564.1 maltose O-acetyltransferase -
AWN69_RS01105 220042..220512 + 471 WP_000136192.1 YlaC family protein -
AWN69_RS01110 220676..222226 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AWN69_RS01115 222268..222621 - 354 WP_000878140.1 DUF1428 domain-containing protein -
AWN69_RS01125 223000..223311 + 312 WP_000409911.1 MGMT family protein -
AWN69_RS01130 223342..223914 - 573 WP_000779842.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T60751 WP_001291435.1 NZ_CP014348:218985-219203 [Escherichia coli str. K-12 substr. MG1655]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T60751 NZ_CP014348:218985-219203 [Escherichia coli str. K-12 substr. MG1655]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT60751 WP_000344800.1 NZ_CP014348:218585-218959 [Escherichia coli str. K-12 substr. MG1655]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT60751 NZ_CP014348:218585-218959 [Escherichia coli str. K-12 substr. MG1655]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References