Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 3008306..3008942 Replicon chromosome
Accession NZ_CP014342
Organism Geobacillus subterraneus strain KCTC3922(T)

Toxin (Protein)


Gene name mazF Uniprot ID L7ZVP1
Locus tag GS3922_RS14560 Protein ID WP_003253417.1
Coordinates 3008306..3008656 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID S5ZZQ7
Locus tag GS3922_RS14565 Protein ID WP_020958454.1
Coordinates 3008661..3008942 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GS3922_RS14550 3004722..3005723 - 1002 WP_063166922.1 LacI family DNA-binding transcriptional regulator -
GS3922_RS14555 3005844..3008006 - 2163 WP_063166923.1 RNA-binding transcriptional accessory protein -
GS3922_RS14560 3008306..3008656 - 351 WP_003253417.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
GS3922_RS14565 3008661..3008942 - 282 WP_020958454.1 hypothetical protein Antitoxin
GS3922_RS14570 3009070..3010224 - 1155 WP_063166924.1 alanine racemase -
GS3922_RS14575 3010514..3011527 - 1014 WP_063166925.1 outer membrane lipoprotein carrier protein LolA -
GS3922_RS14580 3011607..3011996 - 390 WP_063166926.1 holo-ACP synthase -
GS3922_RS14585 3012146..3012763 + 618 WP_063166927.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12992.00 Da        Isoelectric Point: 4.8781

>T60748 WP_003253417.1 NZ_CP014342:c3008656-3008306 [Geobacillus subterraneus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLGLIDF

Download         Length: 351 bp

>T60748 NZ_CP014342:c3008656-3008306 [Geobacillus subterraneus]
TTGATTGTCAAACGTGGCGACGTTTATTTTGCGGACCTTTCCCCGGTCGTTGGCTCAGAGCAGGGCGGCGTACGCCCCGT
GTTGGTGATCCAAAACGATATCGGCAATCGCTTCAGTCCGACGGTAATTGTCGCGGCGATTACGGCGCAAATCCAAAAGG
CGAAACTGCCGACGCACGTCGAAATCGATGCGAAACGCTACGGGTTTGAGCGCGATTCGGTCATTTTGCTTGAGCAAATT
CGTACGATTGATAAACAACGGTTGACCGATAAAATTACCCACTTGGATGATGAAATGATGGATAAGGTCGATGAGGCGCT
GCAAATCAGCTTGGGTTTGATCGACTTTTGA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10641.11 Da        Isoelectric Point: 7.2027

>AT60748 WP_020958454.1 NZ_CP014342:c3008942-3008661 [Geobacillus subterraneus]
VSESGATTEIIVRLPQSLLTELDGLVKQENGNRNELIYQATKMYIRERKKRQIREAMRRGYMEMAKINLSIASEAFHAEY
EADHTVERLVSGG

Download         Length: 282 bp

>AT60748 NZ_CP014342:c3008942-3008661 [Geobacillus subterraneus]
GTGTCGGAATCTGGCGCAACAACGGAAATCATCGTTCGTTTGCCGCAGTCGCTGCTGACAGAACTGGACGGGCTCGTAAA
GCAGGAGAATGGCAATCGCAATGAACTCATTTATCAGGCGACGAAAATGTATATTCGCGAGCGGAAAAAACGGCAAATTC
GCGAGGCGATGAGACGAGGCTACATGGAAATGGCCAAAATCAATTTATCTATTGCTTCTGAAGCGTTTCATGCTGAATAT
GAGGCCGACCACACCGTTGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9ERQ4

Antitoxin

Source ID Structure
AlphaFold DB A0A143ML40

References