Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type VII Classification (family/domain) HepT-MntA/HepT(toxin)
Location 2361300..2362104 Replicon chromosome
Accession NZ_CP014342
Organism Geobacillus subterraneus strain KCTC3922(T)

Toxin (Protein)


Gene name hepT Uniprot ID A0A143MK67
Locus tag GS3922_RS11585 Protein ID WP_063166496.1
Coordinates 2361685..2362104 (+) Length 140 a.a.

Antitoxin (Protein)


Gene name mntA Uniprot ID A0A143MN77
Locus tag GS3922_RS11580 Protein ID WP_063167397.1
Coordinates 2361300..2361695 (+) Length 132 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GS3922_RS11535 2356600..2357082 - 483 WP_020959115.1 DUF111 family protein -
GS3922_RS11540 2357108..2358022 - 915 WP_063166489.1 LarC family nickel insertion protein -
GS3922_RS11545 2358028..2358777 - 750 WP_063167396.1 nickel pincer cofactor biosynthesis protein LarB -
GS3922_RS11550 2358782..2359051 - 270 WP_063166490.1 hypothetical protein -
GS3922_RS11555 2359199..2360002 - 804 WP_063166491.1 ATP-dependent sacrificial sulfur transferase LarE -
GS3922_RS11560 2360030..2360523 - 494 Protein_2315 SDR family oxidoreductase -
GS3922_RS11565 2360545..2360850 - 306 WP_174539025.1 hypothetical protein -
GS3922_RS11570 2360877..2361101 - 225 Protein_2317 beta-glucuronidase -
GS3922_RS17895 2361079..2361237 - 159 WP_156487387.1 hypothetical protein -
GS3922_RS11580 2361300..2361695 + 396 WP_063167397.1 nucleotidyltransferase domain-containing protein Antitoxin
GS3922_RS11585 2361685..2362104 + 420 WP_063166496.1 DUF86 domain-containing protein Toxin
GS3922_RS11590 2362132..2362785 - 654 WP_063166497.1 TetR/AcrR family transcriptional regulator -
GS3922_RS11595 2362797..2363267 - 471 Protein_2322 ABC transporter permease -
GS3922_RS17315 2363195..2363446 - 252 WP_082816565.1 ATP-binding cassette domain-containing protein -
GS3922_RS11600 2363471..2364676 - 1206 WP_063166498.1 DUF3533 domain-containing protein -
GS3922_RS11605 2364814..2365329 - 516 WP_063166499.1 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease HepT-like

(14-131)

Antitoxin

Polymerase beta, nucleotidyltransferase

(5-94)


Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 15933.38 Da        Isoelectric Point: 7.4011

>T60747 WP_063166496.1 NZ_CP014342:2361685-2362104 [Geobacillus subterraneus]
MKSDVILNKISVIERCLKRIREEYNGDPKNLQNYTKQDSIVLNLQRACEACIDLAMHIVAEQKFGLPQHSRDAFALLEEH
GVISPSVSKKMKAMIGFRNIAVHDYQQLNLGIVQAIVEHHLDDFKQFAEAILRYAKENN

Download         Length: 420 bp

>T60747 NZ_CP014342:2361685-2362104 [Geobacillus subterraneus]
ATGAAAAGTGATGTCATTTTAAACAAGATCAGCGTGATCGAACGCTGCCTGAAACGAATTCGTGAAGAATATAACGGCGA
TCCAAAAAATTTACAAAATTACACAAAACAAGATTCGATCGTTCTGAATTTACAGCGGGCGTGCGAAGCCTGCATCGATT
TGGCCATGCATATCGTTGCCGAGCAAAAATTCGGATTGCCGCAGCATAGCCGCGATGCGTTCGCTCTTCTCGAAGAACAT
GGGGTCATCTCCCCTTCCGTAAGCAAAAAGATGAAGGCGATGATCGGATTCCGCAACATCGCTGTTCACGACTACCAACA
ACTGAACCTCGGCATCGTGCAAGCCATCGTTGAACACCATCTTGATGATTTCAAACAGTTTGCCGAAGCTATCCTCCGTT
ATGCGAAAGAAAACAACTAG

Antitoxin


Download         Length: 132 a.a.        Molecular weight: 14789.94 Da        Isoelectric Point: 4.9943

>AT60747 WP_063167397.1 NZ_CP014342:2361300-2361695 [Geobacillus subterraneus]
METVIIQTLRPVLDPFVIYLFGSAARGAMRPDSDVDIAFVSDGEPHDPYELFRLAGHLADKLGRDVDLVDLRQASTVFQA
QVVSTGKAIDCRDERKRAEFEMKTLKMYVKLNEERAPVLKQITESGSIYEK

Download         Length: 396 bp

>AT60747 NZ_CP014342:2361300-2361695 [Geobacillus subterraneus]
ATGGAGACGGTCATCATTCAGACCCTCCGTCCGGTTCTTGACCCGTTCGTCATTTATCTGTTCGGTTCAGCCGCCCGTGG
GGCGATGCGCCCGGACAGTGATGTCGATATCGCCTTTGTCAGCGACGGCGAACCGCATGATCCGTATGAGCTGTTTCGGC
TCGCGGGGCATTTGGCTGACAAGTTAGGGCGAGATGTCGATCTTGTCGATTTGCGCCAAGCCAGCACCGTGTTTCAAGCG
CAAGTCGTCTCGACGGGAAAAGCCATTGATTGCCGCGACGAGCGGAAACGGGCTGAGTTCGAAATGAAAACATTGAAAAT
GTATGTGAAACTAAACGAAGAAAGAGCGCCGGTGTTAAAACAAATTACGGAAAGCGGGAGCATATATGAAAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A143MK67

Antitoxin

Source ID Structure
AlphaFold DB A0A143MN77

References