Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 73554..74079 Replicon plasmid pJJ1887-4
Accession NZ_CP014321
Organism Escherichia coli JJ1887

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag AX202_RS26770 Protein ID WP_001159868.1
Coordinates 73774..74079 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag AX202_RS26765 Protein ID WP_000813634.1
Coordinates 73554..73772 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AX202_RS26740 68833..70446 - 1614 WP_000080195.1 IS66-like element ISEc23 family transposase -
AX202_RS26745 70477..70827 - 351 WP_000624722.1 IS66 family insertion sequence element accessory protein TnpB -
AX202_RS26750 70824..71249 - 426 WP_000422741.1 IS66 family insertion sequence hypothetical protein -
AX202_RS26755 71311..72462 + 1152 WP_031325939.1 DUF3800 domain-containing protein -
AX202_RS26760 72496..73008 - 513 WP_000151784.1 hypothetical protein -
AX202_RS26765 73554..73772 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
AX202_RS26770 73774..74079 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
AX202_RS26775 74080..74886 + 807 WP_000016982.1 site-specific integrase -
AX202_RS26780 75660..76415 + 756 WP_000852146.1 replication initiation protein RepE -
AX202_RS26785 77003..78169 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaOXA-1 / aac(6')-Ib-cr / blaTEM-1B - 1..107507 107507


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T60733 WP_001159868.1 NZ_CP014321:73774-74079 [Escherichia coli JJ1887]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T60733 NZ_CP014321:73774-74079 [Escherichia coli JJ1887]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT60733 WP_000813634.1 NZ_CP014321:73554-73772 [Escherichia coli JJ1887]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT60733 NZ_CP014321:73554-73772 [Escherichia coli JJ1887]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References