Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 66428..67071 Replicon plasmid pJJ1887-4
Accession NZ_CP014321
Organism Escherichia coli JJ1887

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag AX202_RS26720 Protein ID WP_001034046.1
Coordinates 66428..66844 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag AX202_RS26725 Protein ID WP_001261278.1
Coordinates 66841..67071 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AX202_RS26705 61565..61981 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
AX202_RS26710 61978..62208 - 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
AX202_RS26715 62589..66383 + 3795 WP_001144732.1 hypothetical protein -
AX202_RS26720 66428..66844 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
AX202_RS26725 66841..67071 - 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
AX202_RS26730 67336..67836 + 501 WP_000528932.1 hypothetical protein -
AX202_RS26735 67849..68622 + 774 WP_000905949.1 hypothetical protein -
AX202_RS26740 68833..70446 - 1614 WP_000080195.1 IS66-like element ISEc23 family transposase -
AX202_RS26745 70477..70827 - 351 WP_000624722.1 IS66 family insertion sequence element accessory protein TnpB -
AX202_RS26750 70824..71249 - 426 WP_000422741.1 IS66 family insertion sequence hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaOXA-1 / aac(6')-Ib-cr / blaTEM-1B - 1..107507 107507


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T60732 WP_001034046.1 NZ_CP014321:c66844-66428 [Escherichia coli JJ1887]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T60732 NZ_CP014321:c66844-66428 [Escherichia coli JJ1887]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCAGTGCTTAAGAATCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGTC
CAAAGGCATCGCCACGCCACGTGCAGCTGGTTGACGCGTTCTGCGCCCGTCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGATGCAACCACGGAGGTTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGTCCAAACGACACGGCGATCGC
CGGGCACGCCATCGCCACCGGGGCAATACTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCTGGACTGGTACTGG
AAGACTGGGCAGGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT60732 WP_001261278.1 NZ_CP014321:c67071-66841 [Escherichia coli JJ1887]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT60732 NZ_CP014321:c67071-66841 [Escherichia coli JJ1887]
ATGAGAACCGTATCTATTTTCAAAAATGGCAATAACCGCGCGATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
TGAGCTGGAGATTGTACGGGAAGGGGACAGAATCATTCTGCGTCCCCTCCGCCCGAGCTGGAGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGGCTTTATGACGGAACGCGAGGACGTTGTCTGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References