Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 54555..55114 Replicon plasmid pJJ1887-4
Accession NZ_CP014321
Organism Escherichia coli JJ1887

Toxin (Protein)


Gene name relE Uniprot ID V0SVC8
Locus tag AX202_RS26665 Protein ID WP_000421272.1
Coordinates 54839..55114 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0SRR3
Locus tag AX202_RS26660 Protein ID WP_001387467.1
Coordinates 54555..54839 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AX202_RS26630 50018..50458 - 441 Protein_63 zinc-binding dehydrogenase -
AX202_RS26635 50432..50626 + 195 WP_000796505.1 hypothetical protein -
AX202_RS26640 50859..51749 + 891 WP_001077068.1 dihydrodipicolinate synthase family protein -
AX202_RS26645 51774..52154 + 381 WP_000710783.1 Rid family detoxifying hydrolase -
AX202_RS26650 52187..53152 + 966 WP_001022265.1 2-keto-3-deoxygluconate transporter -
AX202_RS26655 53198..53950 - 753 WP_000562172.1 IclR family transcriptional regulator -
AX202_RS26660 54555..54839 + 285 WP_001387467.1 ribbon-helix-helix domain-containing protein Antitoxin
AX202_RS26665 54839..55114 + 276 WP_000421272.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AX202_RS26670 55220..55513 + 294 WP_001105060.1 hypothetical protein -
AX202_RS30305 55548..55676 - 129 Protein_72 IS481 family transposase -
AX202_RS26675 55709..56878 - 1170 WP_001072355.1 hypothetical protein -
AX202_RS26680 57371..57678 + 308 Protein_74 type II toxin-antitoxin system ParD family antitoxin -
AX202_RS26685 57967..58664 + 698 Protein_75 IS1 family transposase -
AX202_RS26695 58918..59940 - 1023 WP_000361402.1 helicase UvrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaOXA-1 / aac(6')-Ib-cr / blaTEM-1B - 1..107507 107507
- flank IS/Tn - - 58161..58664 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 10.0871

>T60730 WP_000421272.1 NZ_CP014321:54839-55114 [Escherichia coli JJ1887]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T60730 NZ_CP014321:54839-55114 [Escherichia coli JJ1887]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATTTGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10460.87 Da        Isoelectric Point: 5.6558

>AT60730 WP_001387467.1 NZ_CP014321:54555-54839 [Escherichia coli JJ1887]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Download         Length: 285 bp

>AT60730 NZ_CP014321:54555-54839 [Escherichia coli JJ1887]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SVC8

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y880

References