Detailed information of TA system TA060729

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/ElaA-DUF1778
• Location: 92419..93222
• Replicon: plasmid pJJ1887-5
• Accession: NZ_CP014320
• Organism: Escherichia coli JJ1887

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: G3CAI8
• Locus tag: AX202_RS26075
• Protein ID: WP_000348883.1
• Coordinates: 92419..92949 (-)
• Length: 177 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: G3CAI7
• Locus tag: AX202_RS26080
• Protein ID: WP_001275013.1
• Coordinates: 92953..93222 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AX202_RS26055	87906..88391	-	486	WP_001403958.1	hypothetical protein	-
AX202_RS26060	88782..91403	+	2622	WP_001586230.1	hypothetical protein	-
AX202_RS26065	91602..91832	+	231	WP_001261274.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
AX202_RS26070	91921..92196	+	276	WP_001403920.1	type II toxin-antitoxin system VapC family toxin	-
AX202_RS30255	92201..92418	-	218	Protein_104	transposase	-
AX202_RS26075	92419..92949	-	531	WP_000348883.1	GNAT family N-acetyltransferase	Toxin
AX202_RS26080	92953..93222	-	270	WP_001275013.1	DUF1778 domain-containing protein	Antitoxin
AX202_RS26085	94110..95099	+	990	WP_123002471.1	RepB family plasmid replication initiator protein	-
AX202_RS26090	95422..96162	-	741	WP_001586233.1	tyrosine-type recombinase/integrase	-
AX202_RS30260	96283..96465	-	183	WP_042004909.1	hypothetical protein	-
AX202_RS26100	96532..96693	-	162	WP_001440647.1	type II toxin-antitoxin system RelE/ParE family toxin	-
AX202_RS26105	96809..97077	-	269	Protein_111	type II toxin-antitoxin system ParD family antitoxin	-
AX202_RS26110	97373..98112	-	740	Protein_112	tyrosine-type recombinase/integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(B) / blaTEM-1B / aac(3)-IIa / mph(A) / sul1 / qacE / dfrB4 / qepA1	-	1..130603	130603

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (92-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 177 a.a.
• Molecular weight: 20321.20 Da
• Isoelectric Point: 6.4889

>T60729 WP_000348883.1 NZ_CP014320:c92949-92419 [Escherichia coli JJ1887]
MDGLRIEIFSEEVEYQLSNFDCGEEYLNTFLTDHLQRQHSSKILRGYLLVTREDRPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCDSL
FYPTKSIEVLFEVNDE

Nucleotide

• Length: 531 bp

>T60729 NZ_CP014320:c92949-92419 [Escherichia coli JJ1887]
ATGGACGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATCAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTCACCGATCATCTGCAACGTCAGCACAGTAGTAAGATTTTACGGGGTTATCTGCTCGTGACGAGAGAGG
ATAGACCACGTGTCATGGGGTACTACACCCTGTCAGGCAGTTGCTTTGAGAAAATACTTCTCCCCAGCAAAACACAACAA
AAGCGTGTGCCCTACAAAAATGTGCCGAGCGTGACTTTGGGCCGTCTGGCAATTGATAAAAGCATTCATCACCAGGGATA
TGGAGAAACACTTGTTACCCATGCGATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGTATACACGGAATGTTCGTGG
AGGCGCTGAACGATAACGCCAAAAAATTCTACCTTCGACTGGGTTTTATACAGTTAAAGGAAGAAAACTGTGATTCGCTC
TTCTACCCGACAAAATCTATTGAAGTATTGTTCGAAGTAAATGACGAATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10229.63 Da
• Isoelectric Point: 6.4702

>AT60729 WP_001275013.1 NZ_CP014320:c93222-92953 [Escherichia coli JJ1887]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASARAAEVIDQHRRLLLNEESWNLVMDAITNPPAPNDRLKR
ATNRLRELE

Nucleotide

• Length: 270 bp

>AT60729 NZ_CP014320:c93222-92953 [Escherichia coli JJ1887]
ATGTCAGCACTGAAGAAGCAGCGCATCGATCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCCATTTCGCAGTTCATGGTCAGTACCGCCTCAGCACGTGCTGCTGAAGTTATTGATCAGCACCGTCGCC
TGCTCCTCAATGAGGAGTCCTGGAATCTGGTTATGGATGCAATCACTAATCCGCCAGCACCAAATGACCGGCTGAAACGC
GCCACTAACCGTCTCCGGGAACTGGAGTAA

Structures

Toxin

• AlphaFold DB: G3CAI8

Antitoxin

• AlphaFold DB: G3CAI7