Detailed information of TA system TA060728

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 91602..92196
• Replicon: plasmid pJJ1887-5
• Accession: NZ_CP014320
• Organism: Escherichia coli JJ1887

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: AX202_RS26070
• Protein ID: WP_001403920.1
• Coordinates: 91921..92196 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: G3CAJ1
• Locus tag: AX202_RS26065
• Protein ID: WP_001261274.1
• Coordinates: 91602..91832 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AX202_RS30335	87105..87877	-	773	Protein_99	hypothetical protein	-
AX202_RS26055	87906..88391	-	486	WP_001403958.1	hypothetical protein	-
AX202_RS26060	88782..91403	+	2622	WP_001586230.1	hypothetical protein	-
AX202_RS26065	91602..91832	+	231	WP_001261274.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AX202_RS26070	91921..92196	+	276	WP_001403920.1	type II toxin-antitoxin system VapC family toxin	Toxin
AX202_RS30255	92201..92418	-	218	Protein_104	transposase	-
AX202_RS26075	92419..92949	-	531	WP_000348883.1	GNAT family N-acetyltransferase	-
AX202_RS26080	92953..93222	-	270	WP_001275013.1	DUF1778 domain-containing protein	-
AX202_RS26085	94110..95099	+	990	WP_123002471.1	RepB family plasmid replication initiator protein	-
AX202_RS26090	95422..96162	-	741	WP_001586233.1	tyrosine-type recombinase/integrase	-
AX202_RS30260	96283..96465	-	183	WP_042004909.1	hypothetical protein	-
AX202_RS26100	96532..96693	-	162	WP_001440647.1	type II toxin-antitoxin system RelE/ParE family toxin	-
AX202_RS26105	96809..97077	-	269	Protein_111	type II toxin-antitoxin system ParD family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(B) / blaTEM-1B / aac(3)-IIa / mph(A) / sul1 / qacE / dfrB4 / qepA1	-	1..130603	130603

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• SpoVT-AbrB domain (7-45)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 9621.03 Da
• Isoelectric Point: 8.5444

>T60728 WP_001403920.1 NZ_CP014320:91921-92196 [Escherichia coli JJ1887]
VFSHRIVVSAITYSEMRFGASGPKASPRHVQLVDKFCPCLDAILSWDRAAVDGTTDIKVALRQAGTPIGQNDTAIAGPEA
GRLGKLTSPTL

Nucleotide

• Length: 276 bp

>T60728 NZ_CP014320:91921-92196 [Escherichia coli JJ1887]
GTGTTTAGTCACCGCATTGTGGTCTCGGCCATTACTTACTCTGAAATGCGCTTCGGTGCCTCCGGTCCGAAGGCCTCCCC
GCGTCACGTGCAACTGGTTGATAAATTCTGCCCGTGCCTCGATGCCATCCTGTCTTGGGACCGCGCCGCGGTGGACGGCA
CCACGGATATTAAAGTGGCACTGCGCCAGGCCGGGACGCCGATCGGCCAGAACGATACGGCGATTGCCGGGCCTGAAGCT
GGAAGACTGGGTAAATTAACATCGCCCACACTCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8856.95 Da
• Isoelectric Point: 4.6286

>AT60728 WP_001261274.1 NZ_CP014320:91602-91832 [Escherichia coli JJ1887]
MRTVSIFKNGNNRAIHLPRDLDFERVRELEIVREGDSIILRPVQPNWGSFAQLEKADPDFMAERENVVSDEGRFDL

Nucleotide

• Length: 231 bp

>AT60728 NZ_CP014320:91602-91832 [Escherichia coli JJ1887]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATCCATCTGCCCCGCGATTTGGATTTTGAGCGGGTGAG
AGAGCTGGAGATCGTCCGCGAAGGAGACAGCATCATCCTGCGTCCCGTCCAGCCGAACTGGGGCTCATTCGCGCAGCTCG
AAAAAGCCGATCCGGATTTTATGGCGGAGCGCGAGAATGTTGTCAGCGACGAAGGTCGATTTGACCTGTGA

Structures

Antitoxin

• AlphaFold DB: G3CAJ1