Detailed information of TA system TA060713

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3403907..3404741
• Replicon: chromosome
• Accession: NZ_CP014316
• Organism: Escherichia coli JJ1887

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PLF5
• Locus tag: AX202_RS17205
• Protein ID: WP_000854690.1
• Coordinates: 3404364..3404741 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1P7N8
• Locus tag: AX202_RS17200
• Protein ID: WP_001305076.1
• Coordinates: 3403907..3404275 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AX202_RS17160	3398989..3400116	+	1128	Protein_3281	hypothetical protein	-
AX202_RS17165	3400192..3400647	+	456	WP_000581502.1	hypothetical protein	-
AX202_RS17170	3400726..3400959	+	234	WP_000902034.1	DUF905 family protein	-
AX202_RS17175	3401060..3401878	+	819	WP_001234620.1	DUF945 domain-containing protein	-
AX202_RS17180	3401933..3402418	+	486	WP_000849565.1	antirestriction protein	-
AX202_RS17185	3402434..3402910	+	477	WP_001186726.1	RadC family protein	-
AX202_RS17190	3402973..3403194	+	222	WP_000692329.1	DUF987 domain-containing protein	-
AX202_RS17195	3403213..3403857	+	645	WP_000094916.1	hypothetical protein	-
AX202_RS17200	3403907..3404275	+	369	WP_001305076.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AX202_RS17205	3404364..3404741	+	378	WP_000854690.1	TA system toxin CbtA family protein	Toxin
AX202_RS17210	3404738..3405226	+	489	WP_000761699.1	hypothetical protein	-
AX202_RS17215	3405243..3405440	+	198	WP_000839293.1	DUF957 domain-containing protein	-
AX202_RS17220	3405525..3406370	+	846	WP_001529401.1	DUF4942 domain-containing protein	-
AX202_RS17225	3406439..3406834	+	396	WP_000208382.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
AX202_RS17230	3406827..3407760	+	934	Protein_3295	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
AX202_RS30375	3408192..3408347	+	156	WP_000729638.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	kpsF / kpsE / kpsD / kpsU / kpsC / kpsS / kpsT	3362170..3422317	60147
	-	flank	IS/Tn	-	-	3408192..3408347	155

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14057.04 Da
• Isoelectric Point: 9.1510

>T60713 WP_000854690.1 NZ_CP014316:3404364-3404741 [Escherichia coli JJ1887]
MKTLPDTHVRAASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYAQVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T60713 NZ_CP014316:3404364-3404741 [Escherichia coli JJ1887]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCAGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13560.39 Da
• Isoelectric Point: 4.7830

>AT60713 WP_001305076.1 NZ_CP014316:3403907-3404275 [Escherichia coli JJ1887]
MSDTLPGTTLPDDNKDLPWWGLPCTVTPCFGACLVQEGNRLHYLADRAGIRGRFSDADAYHPDQAFPLLMKQPELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT60713 NZ_CP014316:3403907-3404275 [Escherichia coli JJ1887]
ATGTCGGACACACTCCCCGGAACAACGCTTCCCGACGACAACAAAGACCTCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCCTGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCGGATGCGTACCATCCGGACCAGGCCTTTCCGCTGCTGATGAAACAACCGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: S1PLF5

Antitoxin

• AlphaFold DB: S1P7N8