Detailed information of TA system TA060711

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rnlAB/RnlA-RnlB
• Location: 2929195..2930620
• Replicon: chromosome
• Accession: NZ_CP014316
• Organism: Escherichia coli JJ1887

Toxin (Protein)

• Gene name: rnlA
• Uniprot ID: -
• Locus tag: AX202_RS14800
• Protein ID: WP_024190590.1
• Coordinates: 2929550..2930620 (-)
• Length: 357 a.a.

Antitoxin (Protein)

• Gene name: rnlB
• Uniprot ID: E6BSF0
• Locus tag: AX202_RS14795
• Protein ID: WP_000461705.1
• Coordinates: 2929195..2929557 (-)
• Length: 121 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AX202_RS14760	2924327..2925988	+	1662	WP_000880939.1	DNA repair protein RecN	-
AX202_RS14765	2926137..2926478	+	342	WP_001203437.1	outer membrane protein assembly factor BamE	-
AX202_RS14770	2926540..2926830	-	291	WP_001117834.1	RnfH family protein	-
AX202_RS14775	2926820..2927257	-	438	WP_001339419.1	type II toxin-antitoxin system toxin RatA	-
AX202_RS14780	2927428..2927910	+	483	WP_000162574.1	SsrA-binding protein SmpB	-
AX202_RS14790	2928788..2929057	+	270	WP_000429347.1	hypothetical protein	-
AX202_RS14795	2929195..2929557	-	363	WP_000461705.1	type II toxin-antitoxin system RnlB family antitoxin	Antitoxin
AX202_RS14800	2929550..2930620	-	1071	WP_024190590.1	type II toxin-antitoxin system RnlA family toxin	Toxin
AX202_RS27860	2930680..2930835	-	156	WP_001597763.1	hypothetical protein	-
AX202_RS14810	2931021..2931605	-	585	WP_023363231.1	tail fiber assembly protein	-
AX202_RS27865	2931605..2934631	-	3027	WP_023363234.1	phage tail protein	-
AX202_RS14830	2934696..2935295	-	600	WP_023363237.1	Ail/Lom family outer membrane beta-barrel protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain (98-183)

Antitoxin

• Antitoxin RnlB/LsoB (15-112)

Sequences

Toxin

Protein

• Length: 357 a.a.
• Molecular weight: 40475.40 Da
• Isoelectric Point: 7.2332

>T60711 WP_024190590.1 NZ_CP014316:c2930620-2929550 [Escherichia coli JJ1887]
MTVRNYTNLNLDRTTIEATSRAFIENKNYSVHSIGPMPGARAGLRVVFAKPGEALATVNIFYNNGGTSTVQYQTGANRNL
GKELADNLYETINPAEFEQVNMVLQGFVEANVLSVLQLSAEQPHIQFYEYLRNTHTTVWKIHSPEFQDELTVSLHHRNGT
LQIQGRPLSCYRVFIFNLSELLDLQGLEKVLIRQDDGKAYIVQKEVARSHLECEMGDAYPLLHKNVEKLLVSGLCVKLAA
PDLPDYCMLLYPELRSVEGVLKSTMYRYGMPITQDGFGPYFDKIGSEFILKQQFGRNLSSAKVKTINDAYTFFNRERHGL
FHMEIVVDTSRMVSDMARLMTKARQAWGIIKDLYIV

Nucleotide

• Length: 1071 bp

>T60711 NZ_CP014316:c2930620-2929550 [Escherichia coli JJ1887]
ATGACAGTCAGGAACTACACTAATTTAAATTTAGATCGGACAACTATTGAGGCTACGTCTAGGGCATTTATTGAAAATAA
AAACTATTCCGTCCATTCAATTGGACCAATGCCTGGTGCAAGAGCAGGACTTCGGGTTGTGTTTGCTAAACCAGGAGAAG
CTTTGGCAACTGTTAACATTTTTTATAATAACGGAGGGACGTCTACAGTCCAGTATCAAACAGGAGCTAATCGTAATCTT
GGAAAAGAGTTGGCTGATAATCTCTATGAAACCATCAATCCTGCAGAATTTGAACAGGTAAATATGGTGTTACAAGGGTT
CGTTGAAGCGAACGTTTTATCTGTACTTCAGCTCTCAGCAGAGCAGCCACATATACAATTTTACGAATACTTACGGAACA
CTCATACAACGGTGTGGAAAATTCATTCGCCAGAATTTCAGGATGAGTTGACAGTTAGTCTGCATCACAGAAATGGAACA
CTACAAATACAAGGGCGTCCATTGTCATGTTATAGAGTGTTTATATTCAACTTGTCAGAGCTACTCGATTTACAAGGACT
GGAAAAAGTTCTCATTCGTCAGGACGATGGCAAAGCGTATATAGTTCAGAAAGAAGTTGCTCGCTCACATCTAGAGTGTG
AAATGGGGGATGCGTACCCACTATTGCATAAGAATGTTGAAAAATTGCTCGTTTCTGGGCTGTGTGTTAAACTCGCCGCC
CCCGATTTACCCGATTACTGTATGTTGTTGTATCCTGAATTACGTTCAGTTGAGGGAGTACTTAAATCAACAATGTACCG
ATATGGCATGCCAATCACGCAGGATGGTTTCGGGCCTTATTTTGATAAAATTGGGAGTGAATTTATTCTGAAACAACAAT
TTGGACGCAATTTATCGTCAGCTAAGGTTAAGACCATTAATGATGCCTATACTTTCTTTAACAGAGAACGTCATGGCCTG
TTTCATATGGAGATAGTTGTCGACACAAGCCGTATGGTTTCTGACATGGCACGACTGATGACTAAGGCCAGACAGGCATG
GGGAATTATTAAGGATTTGTATATTGTTTGA

Antitoxin

Protein

• Length: 121 a.a.
• Molecular weight: 13304.23 Da
• Isoelectric Point: 4.2386

>AT60711 WP_000461705.1 NZ_CP014316:c2929557-2929195 [Escherichia coli JJ1887]
MFEITGVNVSGALIAVVMATGYENPLTSVHEIEKRLSVILGNSQATGEVLFDLLCSNGPEWNRFVSLEIRCGSILLDTAK
IVDEQEIPAQILRHQRDVLRNHPEYVEDSVLTPDDVLCIL

Nucleotide

• Length: 363 bp

>AT60711 NZ_CP014316:c2929557-2929195 [Escherichia coli JJ1887]
TTGTTTGAAATAACAGGTGTTAACGTCTCAGGGGCTCTCATTGCCGTCGTTATGGCGACTGGCTACGAGAACCCCTTGAC
TTCAGTTCACGAAATCGAGAAGAGGCTTTCTGTTATACTGGGCAATTCACAGGCTACAGGTGAGGTTTTGTTTGACCTTT
TGTGTTCTAATGGTCCAGAGTGGAATCGCTTTGTTTCGCTGGAAATAAGGTGTGGCAGTATTTTGCTGGATACTGCCAAA
ATTGTGGATGAGCAAGAAATTCCAGCGCAGATCTTGAGACATCAGAGAGATGTATTACGTAACCACCCTGAGTACGTAGA
AGACTCTGTGTTAACACCAGATGATGTTCTTTGTATTCTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A1X9TDS3