Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 1813465..1813936 Replicon chromosome
Accession NZ_CP014314
Organism Escherichia coli O157:H7 strain JEONG-1266

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag JEONG1266_RS09600 Protein ID WP_001303511.1
Coordinates 1813465..1813743 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag JEONG1266_RS09605 Protein ID WP_001302048.1
Coordinates 1813745..1813936 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JEONG1266_RS09570 1810400..1811485 - 1086 Protein_1821 exonuclease -
JEONG1266_RS09575 1811578..1811769 - 192 WP_001090200.1 DUF1482 family protein -
JEONG1266_RS09580 1811766..1811954 - 189 WP_000449192.1 cell division inhibition protein DicB -
JEONG1266_RS09585 1812523..1812741 - 219 WP_001171930.1 hypothetical protein -
JEONG1266_RS09590 1812813..1813112 - 300 WP_001240334.1 hypothetical protein -
JEONG1266_RS09600 1813465..1813743 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JEONG1266_RS09605 1813745..1813936 - 192 WP_001302048.1 hypothetical protein Antitoxin
JEONG1266_RS31375 1813957..1814328 - 372 WP_001169686.1 hypothetical protein -
JEONG1266_RS09615 1814426..1814728 + 303 WP_000172738.1 transcriptional regulator -
JEONG1266_RS09620 1814725..1815150 + 426 WP_000693943.1 Rha family transcriptional regulator -
JEONG1266_RS09625 1815173..1816135 + 963 WP_000095669.1 helix-turn-helix domain-containing protein -
JEONG1266_RS09630 1816142..1816882 + 741 WP_000788938.1 ATP-binding protein -
JEONG1266_RS09640 1816908..1817677 + 770 Protein_1833 DUF1627 domain-containing protein -
JEONG1266_RS09645 1817693..1818088 + 396 WP_001118159.1 DUF977 family protein -
JEONG1266_RS09650 1818145..1818729 + 585 WP_000206793.1 DUF551 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - nleG7' 1797272..1902010 104738


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T60669 WP_001303511.1 NZ_CP014314:c1813743-1813465 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T60669 NZ_CP014314:c1813743-1813465 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT60669 WP_001302048.1 NZ_CP014314:c1813936-1813745 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT60669 NZ_CP014314:c1813936-1813745 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References