Detailed information of TA system TA060658

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 97399..98033
• Replicon: plasmid unnamed4
• Accession: NZ_CP014311
• Organism: Burkholderia sp. PAMC 26561

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A109WT12
• Locus tag: AXG89_RS34020
• Protein ID: WP_062175022.1
• Coordinates: 97635..98033 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: VapB1
• Uniprot ID: A0A109WXE8
• Locus tag: AXG89_RS34015
• Protein ID: WP_062175021.1
• Coordinates: 97399..97635 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AXG89_RS33985	92497..92814	+	318	WP_062175017.1	hypothetical protein	-
AXG89_RS33990	92848..93243	+	396	WP_062175018.1	hypothetical protein	-
AXG89_RS33995	93368..94920	+	1553	Protein_101	IS3 family transposase	-
AXG89_RS34000	94939..95682	+	744	WP_062175019.1	hypothetical protein	-
AXG89_RS34005	95757..96512	+	756	WP_062175020.1	DUF4393 domain-containing protein	-
AXG89_RS42605	96609..96785	+	177	WP_162916206.1	hypothetical protein	-
AXG89_RS34010	96803..97093	+	291	WP_162916207.1	GNAT family N-acetyltransferase	-
AXG89_RS34015	97399..97635	+	237	WP_062175021.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AXG89_RS34020	97635..98033	+	399	WP_062175022.1	type II toxin-antitoxin system VapC family toxin	Toxin
AXG89_RS34025	98081..98380	+	300	WP_162916208.1	hypothetical protein	-
AXG89_RS34030	98433..98663	+	231	WP_062175024.1	hypothetical protein	-
AXG89_RS42610	98666..98866	+	201	WP_162916209.1	hypothetical protein	-
AXG89_RS34035	99203..99487	+	285	WP_162916210.1	hypothetical protein	-
AXG89_RS34040	99818..101086	+	1269	WP_062173550.1	IS256 family transposase	-
AXG89_RS42895	101462..101641	+	180	WP_205583105.1	hypothetical protein	-
AXG89_RS34050	102042..102440	-	399	WP_062175027.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..184640	184640
	-	flank	IS/Tn	-	-	99818..101086	1268

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-116)

Antitoxin

• SpoVT-AbrB domain (7-44)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14678.05 Da
• Isoelectric Point: 7.7069

>T60658 WP_062175022.1 NZ_CP014311:97635-98033 [Burkholderia sp. PAMC 26561]
MPRYMLDTNMCIYLMKNQPEQVARRFAQCYVGDVVMSAITFAELEYGVVVCANPARERRNLASLIDLIKVVPFAAAAATA
YGPIREATRERKKDQLDKLIAAHAVSLGVPVVTNNVRDFAAYPGVTVENWLD

Nucleotide

• Length: 399 bp

>T60658 NZ_CP014311:97635-98033 [Burkholderia sp. PAMC 26561]
ATGCCGCGTTACATGCTGGACACCAACATGTGCATCTACCTAATGAAGAACCAGCCCGAGCAAGTCGCGCGGCGGTTTGC
TCAGTGCTACGTCGGGGACGTGGTTATGTCAGCGATCACATTTGCCGAACTGGAATACGGCGTCGTTGTCTGTGCGAACC
CGGCACGCGAGCGACGCAACCTTGCATCCCTGATCGACCTCATCAAGGTAGTGCCCTTTGCCGCGGCTGCTGCAACCGCA
TACGGTCCGATTCGAGAGGCGACGCGCGAGAGGAAGAAGGACCAGCTCGACAAGCTGATAGCGGCGCACGCTGTATCGCT
AGGCGTGCCGGTGGTGACTAACAATGTTCGAGACTTCGCCGCTTATCCAGGCGTCACTGTCGAAAATTGGCTTGATTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8805.87 Da
• Isoelectric Point: 5.1548

>AT60658 WP_062175021.1 NZ_CP014311:97399-97635 [Burkholderia sp. PAMC 26561]
MHTTKVFRNGNSQAVRIPAELAYENIDVEVEIERVGDELRIRPARRSLAGVLDRFAKFGTDFMAEGRGSQEQAEREGL

Nucleotide

• Length: 237 bp

>AT60658 NZ_CP014311:97399-97635 [Burkholderia sp. PAMC 26561]
ATGCATACCACCAAGGTTTTCCGCAACGGCAATTCGCAGGCTGTTCGCATCCCCGCCGAGTTGGCTTACGAGAACATCGA
CGTTGAAGTTGAGATTGAGCGCGTGGGCGACGAACTGCGCATTCGCCCCGCGCGTCGCTCACTCGCGGGCGTGCTCGACA
GGTTTGCCAAGTTCGGAACGGACTTCATGGCCGAAGGCCGCGGGAGCCAAGAGCAAGCAGAACGTGAGGGTTTGTAA

Structures

Toxin

• AlphaFold DB: A0A109WT12

Antitoxin

• AlphaFold DB: A0A109WXE8