Detailed information of TA system TA060655

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 118597..119282
• Replicon: plasmid unnamed3
• Accession: NZ_CP014310
• Organism: Burkholderia sp. PAMC 26561

Toxin (Protein)

• Gene name: tad
• Uniprot ID: A0A109WX55
• Locus tag: AXG89_RS31060
• Protein ID: WP_062174884.1
• Coordinates: 118917..119282 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: A0A109WUF4
• Locus tag: AXG89_RS31055
• Protein ID: WP_062174010.1
• Coordinates: 118597..118920 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AXG89_RS31030	113601..114365	-	765	WP_062174348.1	SDR family oxidoreductase	-
AXG89_RS31035	114503..114784	+	282	Protein_118	LysR family transcriptional regulator	-
AXG89_RS31040	115297..116670	+	1374	WP_162916161.1	hypothetical protein	-
AXG89_RS31045	116692..117258	-	567	WP_062174013.1	hypothetical protein	-
AXG89_RS31050	117938..118312	+	375	WP_062174354.1	hypothetical protein	-
AXG89_RS31055	118597..118920	-	324	WP_062174010.1	helix-turn-helix domain-containing protein	Antitoxin
AXG89_RS31060	118917..119282	-	366	WP_062174884.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AXG89_RS31065	119395..119772	-	378	WP_144029411.1	hypothetical protein	-
AXG89_RS31070	119762..119953	-	192	Protein_125	IS3 family transposase	-
AXG89_RS31075	120249..120959	+	711	WP_062174358.1	translesion DNA synthesis-associated protein ImuA	-
AXG89_RS31080	120979..121308	+	330	WP_144029410.1	hypothetical protein	-
AXG89_RS42500	121448..121746	-	299	Protein_128	H-NS histone family protein	-
AXG89_RS31090	121939..122340	+	402	WP_069638450.1	hypothetical protein	-
AXG89_RS31095	122464..123423	-	960	Protein_130	SOS response-associated peptidase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..601076	601076
	-	flank	IS/Tn	-	-	119732..119953	221

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (18-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (30-85)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13416.59 Da
• Isoelectric Point: 9.7653

>T60655 WP_062174884.1 NZ_CP014310:c119282-118917 [Burkholderia sp. PAMC 26561]
MNGMENIKTLRWIASSKKDLKAMPEVVQDTFGFALHQAQIGLKHVDAKALKGFGSAGVLEVVESAEGGTYRAVYTVRFAD
AVYVLHCFQKKATHGIATPKPDMDLIRDRLKIAEELAKERK

Nucleotide

• Length: 366 bp

>T60655 NZ_CP014310:c119282-118917 [Burkholderia sp. PAMC 26561]
ATGAACGGTATGGAAAACATCAAGACTCTGCGCTGGATCGCGTCTTCCAAGAAAGACCTGAAAGCGATGCCCGAAGTCGT
ACAAGACACTTTTGGATTCGCGCTGCATCAGGCCCAGATAGGTCTTAAACACGTGGACGCGAAGGCGCTCAAGGGATTTG
GTTCTGCGGGAGTACTTGAAGTGGTCGAATCGGCGGAAGGCGGGACTTACCGGGCGGTCTACACGGTGCGTTTTGCCGAC
GCGGTGTATGTGCTGCATTGCTTTCAAAAGAAAGCGACCCACGGAATCGCGACACCGAAGCCGGATATGGACCTGATCAG
AGATAGACTCAAGATCGCGGAAGAACTTGCCAAGGAAAGGAAATGA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11688.52 Da
• Isoelectric Point: 8.9999

>AT60655 WP_062174010.1 NZ_CP014310:c118920-118597 [Burkholderia sp. PAMC 26561]
MTDYEESSGNVYTDLGLADAEEMLVKAQLASKIGEIIKGRRWTQQQAAEVLGMTQPKLSNLLRGQFRGVSEAKMLECLAK
LGRDVKIVVGPARRSTKPGHVEVVFAA

Nucleotide

• Length: 324 bp

>AT60655 NZ_CP014310:c118920-118597 [Burkholderia sp. PAMC 26561]
ATGACCGACTACGAAGAAAGCAGCGGCAATGTCTATACAGACTTAGGATTGGCCGATGCGGAGGAAATGCTAGTCAAGGC
GCAACTCGCCTCAAAGATTGGCGAGATCATCAAGGGCCGCCGATGGACGCAACAGCAAGCAGCGGAAGTGCTGGGCATGA
CACAACCCAAGCTGTCCAATTTGCTGCGCGGTCAATTCCGTGGTGTTAGTGAAGCGAAGATGCTCGAATGTCTCGCCAAA
CTTGGGCGTGACGTAAAAATCGTTGTCGGCCCGGCACGCCGTTCGACCAAACCGGGCCATGTTGAAGTGGTGTTTGCTGC
ATAG

Structures

Toxin

• AlphaFold DB: A0A109WX55

Antitoxin

• AlphaFold DB: A0A109WUF4