Detailed information of TA system TA060645

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 3382209..3382836
• Replicon: chromosome
• Accession: NZ_CP014306
• Organism: Burkholderia sp. PAMC 26561

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AXG89_RS15540
• Protein ID: WP_086386644.1
• Coordinates: 3382429..3382836 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: AXG89_RS15535
• Protein ID: WP_062170127.1
• Coordinates: 3382209..3382442 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AXG89_RS15515	3378192..3378527	-	336	WP_062170124.1	chaperone NapD	-
AXG89_RS15520	3378579..3378737	-	159	WP_062170125.1	periplasmic nitrate reductase, NapE protein	-
AXG89_RS15525	3379000..3379704	-	705	WP_062170660.1	glutathione S-transferase N-terminal domain-containing protein	-
AXG89_RS15530	3379968..3382145	+	2178	WP_062170126.1	malate synthase G	-
AXG89_RS15535	3382209..3382442	+	234	WP_062170127.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AXG89_RS15540	3382429..3382836	+	408	WP_086386644.1	PIN domain-containing protein	Toxin
AXG89_RS15550	3383149..3385167	-	2019	WP_062170131.1	cation acetate symporter	-
AXG89_RS15555	3385164..3385430	-	267	WP_086386645.1	DUF4212 domain-containing protein	-
AXG89_RS15560	3385628..3387610	-	1983	WP_062170133.1	acetate--CoA ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-116)

Antitoxin

• SpoVT-AbrB domain (5-37)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14778.84 Da
• Isoelectric Point: 4.3603

>T60645 WP_086386644.1 NZ_CP014306:3382429-3382836 [Burkholderia sp. PAMC 26561]
IAMTKVFLDSNVALYLLSSDSAKANRAEGLMAEGGVISVQVLNEVANVTRRKLCMSWDETGDVLDAIRAVCSTEPLTEET
HDTGRRVSERYGLSVYDSMIVAAALLAECNVLYSEDMQNGLMIDQKLRVVNPFTS

Nucleotide

• Length: 408 bp

>T60645 NZ_CP014306:3382429-3382836 [Burkholderia sp. PAMC 26561]
ATCGCAATGACTAAGGTTTTTCTGGACAGCAATGTCGCGCTCTACCTTCTTTCGAGTGATTCGGCCAAGGCAAACCGCGC
CGAAGGCTTGATGGCAGAAGGCGGTGTTATCAGCGTTCAAGTGCTCAACGAAGTCGCGAACGTAACGCGGCGCAAGTTAT
GTATGTCGTGGGACGAGACGGGTGATGTGTTAGATGCAATCCGCGCCGTTTGCAGCACGGAACCGCTGACTGAGGAAACA
CATGACACCGGTCGACGTGTGTCCGAACGGTACGGACTATCTGTTTATGACTCGATGATCGTAGCGGCCGCACTTCTTGC
CGAATGCAATGTGCTGTACTCCGAAGACATGCAGAACGGGTTGATGATCGATCAAAAGTTGCGGGTTGTTAATCCATTCA
CCAGCTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8766.90 Da
• Isoelectric Point: 7.0137

>AT60645 WP_062170127.1 NZ_CP014306:3382209-3382442 [Burkholderia sp. PAMC 26561]
MQVSKWGNSLAIRLPAAVVEALELHEGDDVDVVVAGAREFQIKKKPTSRELLERLRQYRGRLPADFTFSRDDANRND

Nucleotide

• Length: 234 bp

>AT60645 NZ_CP014306:3382209-3382442 [Burkholderia sp. PAMC 26561]
ATGCAAGTATCGAAGTGGGGAAACAGCCTGGCAATCCGGTTGCCCGCCGCTGTTGTCGAGGCTCTTGAGCTTCATGAAGG
CGACGATGTCGACGTTGTCGTTGCAGGCGCGCGCGAATTTCAGATCAAGAAGAAGCCGACCAGCCGCGAGTTGCTTGAAC
GACTACGACAGTATCGGGGGCGATTGCCTGCTGACTTCACTTTCAGCCGCGACGACGCCAATCGCAATGACTAA