Detailed information of TA system TA060643

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 2473226..2473824
• Replicon: chromosome
• Accession: NZ_CP014306
• Organism: Burkholderia sp. PAMC 26561

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A0X8L7F3
• Locus tag: AXG89_RS11385
• Protein ID: WP_061999041.1
• Coordinates: 2473516..2473824 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: AXG89_RS11380
• Protein ID: WP_061999040.1
• Coordinates: 2473226..2473519 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AXG89_RS11365	2469598..2470743	+	1146	WP_061999037.1	PAS domain-containing sensor histidine kinase	-
AXG89_RS11370	2470889..2472388	+	1500	WP_061999038.1	nitrogen regulation protein NR(I)	-
AXG89_RS11375	2472422..2473198	-	777	WP_061999039.1	exodeoxyribonuclease III	-
AXG89_RS11380	2473226..2473519	-	294	WP_061999040.1	putative addiction module antidote protein	Antitoxin
AXG89_RS11385	2473516..2473824	-	309	WP_061999041.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AXG89_RS11390	2474061..2475335	+	1275	WP_062169742.1	amidohydrolase family protein	-
AXG89_RS11395	2475396..2477528	-	2133	WP_061999043.1	M3 family metallopeptidase	-
AXG89_RS11400	2477715..2478578	-	864	WP_062169743.1	bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Uncharacterized protein HI1420 (4-91)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11559.34 Da
• Isoelectric Point: 10.5091

>T60643 WP_061999041.1 NZ_CP014306:c2473824-2473516 [Burkholderia sp. PAMC 26561]
MTNTINQTREFSGWLLSVKDLSARATILVRIKRAAKGNFGDCHDVGESVWEMRIHTGAGYRVYYVRDGLSVYLLLAGGCK
QGQVADIARAKAMWQRIRQERK

Nucleotide

• Length: 309 bp

>T60643 NZ_CP014306:c2473824-2473516 [Burkholderia sp. PAMC 26561]
ATGACCAACACCATCAATCAAACGCGTGAATTTTCAGGTTGGCTGTTGAGCGTAAAAGACCTGAGCGCACGTGCCACGAT
CCTGGTCCGGATCAAGCGCGCAGCGAAGGGTAATTTTGGCGATTGCCACGATGTTGGCGAAAGCGTCTGGGAAATGCGGA
TCCACACCGGCGCGGGGTATCGTGTTTACTATGTGCGCGACGGCCTGAGCGTCTATCTCTTGTTAGCAGGCGGCTGCAAG
CAAGGGCAAGTAGCGGATATCGCCCGAGCGAAGGCCATGTGGCAGCGAATCAGACAGGAACGGAAATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10194.52 Da
• Isoelectric Point: 4.7483

>AT60643 WP_061999040.1 NZ_CP014306:c2473519-2473226 [Burkholderia sp. PAMC 26561]
MNSTQISAFDASDYLDSEEAIAAYLNEALASGDSDILLSALADVVKARGMSKVASDAGVQRESLYKSLSEGAKPRFETIQ
KVAAALGVKLVAVPLHH

Nucleotide

• Length: 294 bp

>AT60643 NZ_CP014306:c2473519-2473226 [Burkholderia sp. PAMC 26561]
ATGAACTCCACTCAAATTTCAGCATTCGACGCATCCGACTATCTCGACAGCGAAGAGGCCATCGCAGCTTATCTGAATGA
AGCGCTTGCTTCGGGCGACTCGGATATTCTGCTCTCGGCGCTTGCCGACGTCGTAAAGGCACGCGGCATGTCGAAGGTCG
CCTCCGACGCCGGCGTGCAGCGCGAAAGCCTTTACAAGTCGCTCTCCGAAGGCGCGAAACCCCGGTTCGAAACCATCCAG
AAAGTAGCGGCCGCGCTGGGCGTCAAACTCGTCGCCGTGCCGCTGCATCACTGA

Structures

Toxin

• AlphaFold DB: A0A0X8L7F3