Detailed information of TA system TA060641

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 1128366..1128903
• Replicon: chromosome
• Accession: NZ_CP014306
• Organism: Burkholderia sp. PAMC 26561

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: AXG89_RS05410
• Protein ID: WP_062000645.1
• Coordinates: 1128634..1128903 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: AXG89_RS05405
• Protein ID: WP_062168374.1
• Coordinates: 1128366..1128650 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AXG89_RS05375	1123862..1124593	-	732	WP_062168370.1	flagellar hook assembly protein FlgD	-
AXG89_RS05380	1124604..1125029	-	426	WP_056356433.1	flagellar basal body rod protein FlgC	-
AXG89_RS05385	1125058..1125549	-	492	WP_062001023.1	flagellar basal body rod protein FlgB	-
AXG89_RS05390	1125785..1127257	+	1473	WP_062168372.1	flagellar basal body P-ring formation protein FlgA	-
AXG89_RS05395	1127447..1127785	+	339	WP_062000642.1	flagellar biosynthesis anti-sigma factor FlgM	-
AXG89_RS05400	1127884..1128327	+	444	WP_062000643.1	flagellar protein FlgN	-
AXG89_RS05405	1128366..1128650	-	285	WP_062168374.1	BrnA antitoxin family protein	Antitoxin
AXG89_RS05410	1128634..1128903	-	270	WP_062000645.1	BrnT family toxin	Toxin
AXG89_RS05415	1129088..1130308	+	1221	WP_062000646.1	flagellar type III secretion system protein FlhB	-
AXG89_RS05420	1130305..1132407	+	2103	WP_062168376.1	flagellar biosynthesis protein FlhA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10417.83 Da
• Isoelectric Point: 7.1644

>T60641 WP_062000645.1 NZ_CP014306:c1128903-1128634 [Burkholderia sp. PAMC 26561]
MDITFDAAKSASNMEKHGVGLDQARQFEWSDVMFYVDTRRDYKELREVGFGVISERLYCVVFTQRGDAMHIISMRKANKR
EVKSYVEQT

Nucleotide

• Length: 270 bp

>T60641 NZ_CP014306:c1128903-1128634 [Burkholderia sp. PAMC 26561]
ATGGACATCACATTTGACGCCGCCAAGAGCGCCAGCAACATGGAAAAGCATGGGGTTGGGCTAGATCAGGCAAGACAATT
CGAGTGGTCGGACGTGATGTTTTATGTCGACACCCGCAGAGACTATAAAGAGTTGCGAGAAGTCGGGTTCGGGGTGATCA
GCGAACGACTTTATTGCGTGGTCTTCACGCAGCGCGGCGACGCAATGCACATCATCAGCATGCGTAAGGCGAACAAGCGA
GAGGTGAAAAGCTATGTCGAACAAACCTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10701.13 Da
• Isoelectric Point: 8.5274

>AT60641 WP_062168374.1 NZ_CP014306:c1128650-1128366 [Burkholderia sp. PAMC 26561]
MSNKPKIIMPTDEEDAAINRGIAADPDTYEVPGEDFKKMKRLGARGRPRAETPKVQLTVRYDADIVDKFKATGDGWQTRM
NDALRDWLQTHRLA

Nucleotide

• Length: 285 bp

>AT60641 NZ_CP014306:c1128650-1128366 [Burkholderia sp. PAMC 26561]
ATGTCGAACAAACCTAAGATCATCATGCCGACCGACGAGGAAGACGCAGCGATCAATCGTGGTATCGCCGCCGATCCGGA
TACCTATGAAGTTCCTGGGGAGGACTTCAAGAAAATGAAGCGCCTGGGTGCGCGCGGCCGGCCACGTGCCGAGACACCCA
AGGTTCAGTTGACTGTTCGCTACGATGCGGACATCGTGGACAAGTTCAAGGCGACCGGCGACGGTTGGCAAACACGCATG
AATGATGCGCTGCGCGACTGGTTGCAGACCCATCGCCTGGCTTGA