Detailed information of TA system TA060630

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-RHH
• Location: 2395129..2395670
• Replicon: chromosome
• Accession: NZ_CP014301
• Organism: Bosea sp. PAMC 26642 strain PAMC26642

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A126NV86
• Locus tag: AXW83_RS11435
• Protein ID: WP_066613558.1
• Coordinates: 2395374..2395670 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A126NV77
• Locus tag: AXW83_RS11430
• Protein ID: WP_066613552.1
• Coordinates: 2395129..2395377 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AXW83_RS11400 (AXW83_11405)	2390389..2390994	+	606	WP_066613542.1	glutathione S-transferase	-
AXW83_RS11405 (AXW83_11410)	2391219..2391923	-	705	WP_066613544.1	porin family protein	-
AXW83_RS11410 (AXW83_11415)	2392296..2393093	+	798	WP_082767073.1	glycosyltransferase family 2 protein	-
AXW83_RS11415 (AXW83_11420)	2393059..2393835	+	777	WP_066613547.1	SDR family oxidoreductase	-
AXW83_RS11420 (AXW83_11425)	2393846..2394475	-	630	WP_066613549.1	2-hydroxychromene-2-carboxylate isomerase	-
AXW83_RS11425 (AXW83_11430)	2394650..2394934	-	285	WP_066613551.1	hypothetical protein	-
AXW83_RS11430 (AXW83_11435)	2395129..2395377	+	249	WP_066613552.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
AXW83_RS11435 (AXW83_11440)	2395374..2395670	+	297	WP_066613558.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AXW83_RS11440 (AXW83_11445)	2395676..2396440	-	765	WP_066613560.1	peptidoglycan editing factor PgeF	-
AXW83_RS11445 (AXW83_11450)	2396491..2397639	-	1149	WP_210179697.1	SAM-dependent methyltransferase	-
AXW83_RS11450 (AXW83_11455)	2397705..2398541	-	837	WP_066613564.1	prolipoprotein diacylglyceryl transferase	-
AXW83_RS11455 (AXW83_11460)	2398685..2398924	+	240	WP_066613566.1	plasmid stability protein	-
AXW83_RS11460 (AXW83_11465)	2398921..2399340	+	420	WP_066613570.1	type II toxin-antitoxin system VapC family toxin	-
AXW83_RS11465 (AXW83_11470)	2399372..2399938	-	567	WP_066620342.1	chloramphenicol phosphotransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-92)

Antitoxin

• Antitoxin ParD (1-80)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11364.11 Da
• Isoelectric Point: 11.7505

>T60630 WP_066613558.1 NZ_CP014301:2395374-2395670 [Bosea sp. PAMC 26642]
MTLRILVRPKAVADLEDIWRFSKSEWGARQADLYLESLRSAFARLADTPGSGAVFEHVRANYQRLLIGSHAIFYRVSSRQ
IDIVRVLHQRRNASRILR

Nucleotide

• Length: 297 bp

>T60630 NZ_CP014301:2395374-2395670 [Bosea sp. PAMC 26642]
ATGACGCTTCGAATCCTCGTCCGGCCGAAAGCCGTGGCCGATCTCGAAGACATATGGCGCTTCTCTAAGAGCGAGTGGGG
CGCGCGGCAGGCAGACCTGTATTTGGAATCGCTACGATCAGCGTTCGCGCGTCTCGCGGATACCCCCGGCAGCGGCGCCG
TTTTCGAACATGTTCGTGCTAATTATCAACGGCTACTCATCGGGTCTCACGCCATCTTCTACAGGGTGTCCAGCCGTCAA
ATCGATATCGTCCGTGTCCTGCACCAGCGCCGCAACGCCTCGCGGATATTGCGCTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8939.76 Da
• Isoelectric Point: 3.8563

>AT60630 WP_066613552.1 NZ_CP014301:2395129-2395377 [Bosea sp. PAMC 26642]
MGENTSISLGDHFVAFIDKQVAEGRYVSASEVVRAALQLLEENEAEIAALQAALIEGEESGDAEEFDFEAFIARMHAEHE
QG

Nucleotide

• Length: 249 bp

>AT60630 NZ_CP014301:2395129-2395377 [Bosea sp. PAMC 26642]
ATGGGCGAGAACACGTCGATCAGCCTGGGCGACCATTTCGTCGCTTTCATCGACAAGCAAGTGGCCGAGGGACGTTATGT
CTCCGCAAGCGAGGTCGTCAGGGCCGCGTTGCAATTGCTGGAAGAGAATGAGGCGGAGATCGCGGCCCTGCAGGCAGCCT
TGATAGAAGGCGAAGAGAGCGGCGATGCCGAGGAGTTCGACTTCGAGGCTTTCATCGCCAGAATGCACGCCGAGCATGAA
CAGGGATGA

Structures

Toxin

• AlphaFold DB: A0A126NV86

Antitoxin

• AlphaFold DB: A0A126NV77