Detailed information of TA system TA060629

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1084380..1085067
• Replicon: chromosome
• Accession: NZ_CP014301
• Organism: Bosea sp. PAMC 26642 strain PAMC26642

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AXW83_RS05090
• Protein ID: WP_066611221.1
• Coordinates: 1084660..1085067 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A126NSF1
• Locus tag: AXW83_RS05085
• Protein ID: WP_066611219.1
• Coordinates: 1084380..1084652 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AXW83_RS05070 (AXW83_05075)	1079878..1080564	+	687	WP_066611215.1	GntR family transcriptional regulator	-
AXW83_RS05075 (AXW83_05080)	1080717..1082024	+	1308	WP_066611217.1	aspartate aminotransferase family protein	-
AXW83_RS05080 (AXW83_05085)	1082587..1084265	+	1679	Protein_1023	methionine--tRNA ligase	-
AXW83_RS05085 (AXW83_05090)	1084380..1084652	+	273	WP_066611219.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AXW83_RS05090 (AXW83_05095)	1084660..1085067	+	408	WP_066611221.1	type II toxin-antitoxin system VapC family toxin	Toxin
AXW83_RS05095 (AXW83_05100)	1085611..1086180	-	570	WP_156639809.1	flavin reductase family protein	-
AXW83_RS05100 (AXW83_05105)	1086194..1087024	-	831	WP_066611223.1	ATP-binding cassette domain-containing protein	-
AXW83_RS05105 (AXW83_05110)	1087035..1087889	-	855	WP_082766952.1	aliphatic sulfonate ABC transporter permease SsuC	-
AXW83_RS05110 (AXW83_05115)	1087897..1089048	-	1152	WP_066611225.1	FMNH2-dependent alkanesulfonate monooxygenase	-
AXW83_RS05115 (AXW83_05120)	1089091..1090050	-	960	WP_066611226.1	sulfonate ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-126)

Antitoxin

• SpoVT-AbrB domain (13-49)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14607.67 Da
• Isoelectric Point: 4.9163

>T60629 WP_066611221.1 NZ_CP014301:1084660-1085067 [Bosea sp. PAMC 26642]
MFCLDTNVVVHALNGRVPAVDARLQQELAVGTRLLISSVVVFELRYGIAKSVRREASGRVLDTFLSEGFEMVPFDADDAA
EAGDIRATLEAAGTPIGAYDTLIAAQARRRGAVLVTGNRREFERVPGLMVTDWAS

Nucleotide

• Length: 408 bp

>T60629 NZ_CP014301:1084660-1085067 [Bosea sp. PAMC 26642]
ATGTTCTGCCTCGATACCAACGTCGTCGTTCATGCCCTGAACGGGCGGGTTCCCGCGGTCGATGCCAGGCTTCAACAGGA
GCTCGCCGTCGGAACCCGGCTTCTCATCTCGTCGGTGGTCGTTTTCGAGCTGAGATATGGGATAGCCAAGAGCGTGAGGC
GTGAGGCCTCAGGCCGCGTACTCGATACGTTTCTCTCCGAAGGATTCGAGATGGTGCCGTTCGATGCCGACGATGCAGCG
GAAGCCGGCGATATTCGGGCCACCCTCGAAGCGGCCGGAACGCCGATCGGCGCCTACGATACTCTGATTGCGGCGCAGGC
CCGGCGGCGCGGTGCGGTTCTGGTCACCGGCAATCGGCGCGAGTTCGAGCGCGTGCCTGGCCTGATGGTGACGGACTGGG
CCTCTTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 9880.11 Da
• Isoelectric Point: 6.7256

>AT60629 WP_066611219.1 NZ_CP014301:1084380-1084652 [Bosea sp. PAMC 26642]
MSARTNSSTAKLFMHGRSQAVRLPKEFRFEGTEVHVSRVGNKVVLEPIRKPAFDAGAWRAQLDALGARDFLTEGLPEDPH
VGADDTVDFG

Nucleotide

• Length: 273 bp

>AT60629 NZ_CP014301:1084380-1084652 [Bosea sp. PAMC 26642]
ATGAGCGCCCGGACGAACTCATCGACCGCCAAGCTCTTCATGCATGGCCGCAGCCAGGCGGTCCGTCTGCCGAAGGAATT
CCGCTTCGAGGGAACGGAGGTCCATGTCAGCAGGGTCGGAAACAAGGTCGTCCTTGAGCCCATCAGGAAGCCTGCCTTCG
ACGCCGGCGCCTGGAGGGCGCAGCTCGATGCGCTGGGCGCCCGCGATTTCCTGACGGAGGGGCTGCCGGAAGATCCGCAT
GTTGGAGCCGACGACACAGTCGATTTCGGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A126NSF1