Detailed information of TA system TA060625

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-Phd
• Location: 95073..95758
• Replicon: chromosome
• Accession: NZ_CP014301
• Organism: Bosea sp. PAMC 26642 strain PAMC26642

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A126NTR0
• Locus tag: AXW83_RS00455
• Protein ID: WP_066609692.1
• Coordinates: 95073..95498 (-)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A126NQ93
• Locus tag: AXW83_RS00460
• Protein ID: WP_066609693.1
• Coordinates: 95495..95758 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AXW83_RS00435 (AXW83_00435)	90403..91578	+	1176	WP_066609682.1	glycine cleavage system aminomethyltransferase GcvT	-
AXW83_RS00440 (AXW83_00440)	91616..91990	+	375	WP_066609684.1	glycine cleavage system protein GcvH	-
AXW83_RS00445 (AXW83_00445)	92151..93497	+	1347	WP_066609686.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPA	-
AXW83_RS00450 (AXW83_00450)	93491..95065	+	1575	WP_066609690.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPB	-
AXW83_RS00455 (AXW83_00455)	95073..95498	-	426	WP_066609692.1	type II toxin-antitoxin system VapC family toxin	Toxin
AXW83_RS00460 (AXW83_00460)	95495..95758	-	264	WP_066609693.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
AXW83_RS00465 (AXW83_00465)	95932..97173	+	1242	WP_066609698.1	L,D-transpeptidase family protein	-
AXW83_RS00470 (AXW83_00470)	97473..97949	-	477	WP_066609699.1	ATP F0F1 synthase subunit B	-
AXW83_RS00475 (AXW83_00475)	97961..98560	-	600	WP_082766861.1	hypothetical protein	-
AXW83_RS00480 (AXW83_00480)	98656..98883	-	228	WP_066609701.1	F0F1 ATP synthase subunit C	-
AXW83_RS00485 (AXW83_00485)	98947..99708	-	762	WP_066609702.1	F0F1 ATP synthase subunit A	-
AXW83_RS00490 (AXW83_00490)	99754..100104	-	351	WP_066609703.1	AtpZ/AtpI family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-125)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (7-71)

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 15811.16 Da
• Isoelectric Point: 8.7260

>T60625 WP_066609692.1 NZ_CP014301:c95498-95073 [Bosea sp. PAMC 26642]
MTFLLDTNVVSEPQKPRPAAAVERFFRETPERNLYVSVVTTAELYRGLALMTPGHRHFALSRWITEALPRRFGGRLLPVT
VSIAARWGDIMAHSHRTGLNMSIMDGFLAATAAEHDLTLATRNVRHFADLGVPVFNPWDEA

Nucleotide

• Length: 426 bp

>T60625 NZ_CP014301:c95498-95073 [Bosea sp. PAMC 26642]
ATGACGTTCCTGCTCGATACAAACGTCGTCTCGGAACCGCAGAAACCGCGTCCAGCTGCGGCAGTAGAGCGTTTTTTCCG
AGAAACGCCGGAGCGCAACCTCTATGTCAGCGTGGTCACAACCGCAGAACTTTACCGCGGACTTGCGTTGATGACACCGG
GCCATCGCCATTTCGCATTGTCGCGGTGGATTACCGAAGCGCTTCCGCGACGCTTCGGTGGACGGCTCCTGCCCGTCACG
GTTTCAATTGCCGCCCGCTGGGGCGATATCATGGCCCATTCTCACCGCACCGGGCTGAACATGTCCATCATGGACGGATT
CCTGGCGGCAACGGCGGCGGAACACGACCTCACGCTCGCTACCCGCAACGTCCGACATTTCGCTGATCTCGGCGTGCCCG
TCTTCAACCCCTGGGACGAAGCCTGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9796.87 Da
• Isoelectric Point: 4.5386

>AT60625 WP_066609693.1 NZ_CP014301:c95758-95495 [Bosea sp. PAMC 26642]
MSAVSKETWTVASAKARFSEFIESARSDGPQIVTRNGKPAAVLVSSEDWERTASREENLFDFLRNSPLRGADLDLERLDE
KPRDLDL

Nucleotide

• Length: 264 bp

>AT60625 NZ_CP014301:c95758-95495 [Bosea sp. PAMC 26642]
ATGAGCGCAGTTTCAAAGGAAACTTGGACAGTCGCCAGCGCCAAGGCTCGCTTTTCCGAGTTCATCGAGAGCGCGCGGTC
GGATGGACCGCAGATCGTAACCCGGAACGGCAAACCCGCTGCGGTTCTGGTATCGTCCGAGGACTGGGAGCGAACAGCGT
CTCGCGAGGAGAACCTGTTCGATTTCCTGCGGAATTCGCCGTTGCGTGGCGCCGATCTCGATCTGGAGCGGCTAGACGAA
AAGCCGCGCGACCTCGACCTATGA

Structures

Toxin

• AlphaFold DB: A0A126NTR0

Antitoxin

• AlphaFold DB: A0A126NQ93