60622

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 233820..234462 Replicon chromosome
Accession NZ_CP014282
Organism Bacillus thuringiensis strain Bt185

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag AXW78_RS01305 Protein ID WP_000635965.1
Coordinates 234112..234462 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag AXW78_RS01300 Protein ID WP_000004570.1
Coordinates 233820..234107 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AXW78_RS01275 229137..230099 + 963 WP_000961160.1 UV DNA damage repair endonuclease UvsE -
AXW78_RS01280 230092..230664 - 573 WP_000908521.1 rhomboid family intramembrane serine protease -
AXW78_RS01285 230758..231117 + 360 WP_000583417.1 holo-ACP synthase -
AXW78_RS01290 231274..232224 + 951 WP_002004297.1 outer membrane lipoprotein carrier protein LolA -
AXW78_RS01295 232342..233511 + 1170 WP_000390613.1 alanine racemase -
AXW78_RS01300 233820..234107 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
AXW78_RS01305 234112..234462 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
AXW78_RS01310 234530..236698 + 2169 WP_000426236.1 RNA-binding transcriptional accessory protein -
AXW78_RS31445 236756..236872 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
AXW78_RS01315 237068..237526 + 459 WP_061883709.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T60622 WP_000635965.1 NZ_CP014282:234112-234462 [Bacillus thuringiensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T60622 NZ_CP014282:234112-234462 [Bacillus thuringiensis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT60622 WP_000004570.1 NZ_CP014282:233820-234107 [Bacillus thuringiensis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT60622 NZ_CP014282:233820-234107 [Bacillus thuringiensis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References