Detailed information of TA system TA060621

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 7024..7685
• Replicon: plasmid unnamed2
• Accession: NZ_CP014277
• Organism: Martelella sp. AD-3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AZF01_RS22550
• Protein ID: WP_024709692.1
• Coordinates: 7281..7685 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A127CRD0
• Locus tag: AZF01_RS22545
• Protein ID: WP_024709693.1
• Coordinates: 7024..7284 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AZF01_RS22525	2360..3283	+	924	WP_036238132.1	DUF1738 domain-containing protein	-
AZF01_RS22530	3723..4019	+	297	WP_024709696.1	helix-turn-helix domain-containing protein	-
AZF01_RS22535	4162..6279	+	2118	WP_197489684.1	ParB/RepB/Spo0J family partition protein	-
AZF01_RS22540	6406..6963	+	558	WP_024709694.1	hypothetical protein	-
AZF01_RS22545	7024..7284	+	261	WP_024709693.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AZF01_RS22550	7281..7685	+	405	WP_024709692.1	type II toxin-antitoxin system VapC family toxin	Toxin
AZF01_RS22555	7711..8337	+	627	WP_051424147.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..166530	166530

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (15-56)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14004.20 Da
• Isoelectric Point: 5.5857

>T60621 WP_024709692.1 NZ_CP014277:7281-7685 [Martelella sp. AD-3]
MSRLYMLDTNIVSDLARNPAGAVVGHIADVGQDTICVSIITAAELCYGCERKGSAKLTAQVEAILGGMQILALGAPAHAR
YGRIRAGLEAAGTPIGPNDLLIAAHALAVDAVLVTANTREFLRVPGLQVENWLD

Nucleotide

• Length: 405 bp

>T60621 NZ_CP014277:7281-7685 [Martelella sp. AD-3]
TTGAGCCGCCTTTACATGCTCGACACGAATATCGTGTCGGATCTCGCGCGTAATCCGGCCGGCGCCGTCGTCGGCCATAT
CGCTGATGTCGGGCAGGATACGATCTGCGTCAGCATTATCACCGCGGCTGAGCTATGCTATGGCTGCGAACGCAAGGGAT
CTGCCAAGCTGACAGCCCAGGTCGAGGCCATTCTTGGCGGCATGCAAATACTCGCATTGGGTGCGCCAGCACATGCCCGG
TACGGCCGCATCAGAGCAGGCCTCGAAGCAGCCGGTACGCCGATCGGTCCAAATGACCTGCTGATTGCAGCCCATGCCTT
GGCTGTCGATGCTGTGCTGGTAACAGCCAATACGCGCGAATTCCTTCGGGTGCCCGGTCTCCAGGTTGAAAACTGGCTGG
ACTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9642.10 Da
• Isoelectric Point: 5.8433

>AT60621 WP_024709693.1 NZ_CP014277:7024-7284 [Martelella sp. AD-3]
MPQPQASEAREAKLFRNNKSQAVRIPADFELPGDRVLIHREGSRLVIEPAPNKNLLDILARLKPLGPDDQLPDVDETLLP
ARKIDL

Nucleotide

• Length: 261 bp

>AT60621 NZ_CP014277:7024-7284 [Martelella sp. AD-3]
ATGCCCCAGCCCCAAGCGTCCGAAGCACGTGAAGCCAAGTTGTTTCGCAACAACAAGAGTCAGGCGGTTCGTATCCCGGC
CGATTTCGAACTTCCGGGCGACCGTGTGCTGATACATCGGGAGGGAAGCCGACTTGTGATCGAACCTGCCCCAAACAAAA
ATCTTCTGGACATTCTGGCGCGACTGAAGCCGCTCGGCCCTGATGACCAGCTTCCCGACGTCGATGAGACCCTGCTGCCA
GCAAGGAAAATCGACCTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A127CRD0