Detailed information of TA system TA060615

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-MazE
• Location: 2977778..2978391
• Replicon: chromosome
• Accession: NZ_CP014274
• Organism: Stenotrophomonas sp. KCTC 12332 strain YM1

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: -
• Locus tag: AXG53_RS12650
• Protein ID: WP_062168809.1
• Coordinates: 2977778..2978140 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: A0A126NKU1
• Locus tag: AXG53_RS12655
• Protein ID: WP_062168811.1
• Coordinates: 2978134..2978391 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AXG53_RS12635	2974352..2974654	+	303	WP_062168803.1	DUF3892 domain-containing protein	-
AXG53_RS20215	2974696..2975472	-	777	WP_156455855.1	hypothetical protein	-
AXG53_RS12645	2975822..2976778	+	957	WP_062168807.1	hypothetical protein	-
AXG53_RS20220	2977256..2977393	-	138	WP_156455856.1	hypothetical protein	-
AXG53_RS20225	2977390..2977560	-	171	WP_156455857.1	hypothetical protein	-
AXG53_RS12650	2977778..2978140	-	363	WP_062168809.1	type II toxin-antitoxin system ChpB family toxin	Toxin
AXG53_RS12655	2978134..2978391	-	258	WP_062168811.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AXG53_RS12660	2978607..2980958	+	2352	WP_062168814.1	GH92 family glycosyl hydrolase	-
AXG53_RS12665	2981080..2981532	+	453	WP_062168816.1	GNAT family N-acetyltransferase	-
AXG53_RS12670	2981541..2981858	-	318	WP_062168817.1	quaternary ammonium compound efflux SMR transporter SugE	-
AXG53_RS12680	2982542..2983306	-	765	WP_062168819.1	MBL fold metallo-hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2974696..3005470	30774

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-116)

Antitoxin

• Antitoxin PemI-like (1-85)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 12483.29 Da
• Isoelectric Point: 8.9971

>T60615 WP_062168809.1 NZ_CP014274:c2978140-2977778 [Stenotrophomonas sp. KCTC 12332]
VVSRPRFDRGDIIRVQLNPSAGQEMQGDMRPALVLSPAPFNGTGLAIVAPITQGGMVGRNAGFAVSLSATGTETQGVVLV
NHLRSMDLSARGARKVERAPASVVDEALAKVATFLGESVE

Nucleotide

• Length: 363 bp

>T60615 NZ_CP014274:c2978140-2977778 [Stenotrophomonas sp. KCTC 12332]
GTGGTGAGTCGCCCGCGATTTGATCGCGGCGACATCATCCGGGTTCAGCTCAACCCCAGCGCAGGCCAGGAAATGCAGGG
CGACATGCGTCCGGCGCTTGTCCTGAGTCCGGCGCCATTCAACGGCACGGGCTTGGCGATCGTTGCCCCGATCACACAGG
GCGGAATGGTGGGGCGAAATGCGGGGTTCGCGGTTTCGCTGTCAGCGACGGGCACCGAGACGCAGGGCGTGGTCCTGGTC
AACCACCTGCGAAGCATGGATTTGAGCGCGCGCGGTGCACGAAAGGTGGAGCGAGCGCCTGCCAGTGTTGTGGATGAGGC
GTTGGCGAAGGTGGCTACGTTTCTTGGGGAATCGGTGGAGTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9245.51 Da
• Isoelectric Point: 4.6855

>AT60615 WP_062168811.1 NZ_CP014274:c2978391-2978134 [Stenotrophomonas sp. KCTC 12332]
MHAKIQRWGNSAAVRLPASILKAMDLGSGDTLEIGVEDGTLILTPVKVRPRYQLSELLAQCDLSAPQDEELSAWNSSKPV
GNEAW

Nucleotide

• Length: 258 bp

>AT60615 NZ_CP014274:c2978391-2978134 [Stenotrophomonas sp. KCTC 12332]
ATGCACGCCAAAATTCAGCGTTGGGGCAATAGCGCAGCCGTGCGCCTGCCTGCGTCCATTCTCAAAGCCATGGATCTGGG
CAGTGGTGACACGCTCGAGATTGGCGTCGAAGACGGCACGTTGATCCTGACGCCGGTCAAGGTGCGGCCCCGCTATCAGT
TGTCGGAGTTGCTGGCGCAGTGTGATTTGAGCGCCCCGCAGGACGAAGAGCTGTCTGCCTGGAACAGCAGCAAGCCGGTA
GGGAACGAAGCGTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A126NKU1