Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3143678..3144332 Replicon chromosome
Accession NZ_CP014272
Organism Escherichia coli K-12 strain K-12 C3026

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag C3026_RS15855 Protein ID WP_000244777.1
Coordinates 3143678..3144085 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag C3026_RS15860 Protein ID WP_000354046.1
Coordinates 3144066..3144332 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C3026_RS15835 3139635..3141368 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
C3026_RS15840 3141374..3142084 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
C3026_RS15845 3142109..3143005 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
C3026_RS15850 3143117..3143638 + 522 WP_001055874.1 flavodoxin FldB -
C3026_RS15855 3143678..3144085 - 408 WP_000244777.1 toxin CptA Toxin
C3026_RS15860 3144066..3144332 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
C3026_RS15865 3144575..3145555 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
C3026_RS15870 3145751..3146410 - 660 WP_000250274.1 hemolysin III family protein -
C3026_RS15875 3146574..3146885 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
C3026_RS15880 3146930..3148363 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
C3026_RS15885 3148420..3149163 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T60593 WP_000244777.1 NZ_CP014272:c3144085-3143678 [Escherichia coli K-12]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T60593 NZ_CP014272:c3144085-3143678 [Escherichia coli K-12]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT60593 WP_000354046.1 NZ_CP014272:c3144332-3144066 [Escherichia coli K-12]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT60593 NZ_CP014272:c3144332-3144066 [Escherichia coli K-12]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References