Detailed information of TA system TA060577

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1613455..1614093
• Replicon: chromosome
• Accession: NZ_CP014272
• Organism: Escherichia coli K-12 strain K-12 C3026

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: C3026_RS08340
• Protein ID: WP_000813794.1
• Coordinates: 1613455..1613631 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C3026_RS08345
• Protein ID: WP_001270286.1
• Coordinates: 1613677..1614093 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3026_RS08320	1609074..1610288	-	1215	WP_001326689.1	BenE family transporter YdcO	-
C3026_RS08325	1610341..1610877	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
C3026_RS08330	1610950..1612911	+	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
C3026_RS08335	1613003..1613233	-	231	WP_000494244.1	YncJ family protein	-
C3026_RS08340	1613455..1613631	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
C3026_RS08345	1613677..1614093	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
C3026_RS08350	1614172..1615578	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
C3026_RS08355	1615823..1616968	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
C3026_RS08360	1616986..1617999	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
C3026_RS08365	1618000..1618941	+	942	WP_001251304.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T60577 WP_000813794.1 NZ_CP014272:1613455-1613631 [Escherichia coli K-12]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T60577 NZ_CP014272:1613455-1613631 [Escherichia coli K-12]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT60577 WP_001270286.1 NZ_CP014272:1613677-1614093 [Escherichia coli K-12]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT60577 NZ_CP014272:1613677-1614093 [Escherichia coli K-12]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB