Detailed information of TA system TA060556

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 18423..19102
• Replicon: plasmid F128-(DHB4)
• Accession: NZ_CP014271
• Organism: Escherichia coli K-12 strain K-12 DHB4

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: DHB4_RS22665
• Protein ID: WP_000854672.1
• Coordinates: 18423..18764 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: DHB4_RS22670
• Protein ID: WP_000070395.1
• Coordinates: 18785..19102 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DHB4_RS22640	13700..14101	+	402	WP_000174689.1	sigma factor-binding protein Crl	-
DHB4_RS22645	14140..15195	-	1056	WP_000749863.1	phosphoporin PhoE	-
DHB4_RS22650	15483..16586	+	1104	WP_001285288.1	glutamate 5-kinase	-
DHB4_RS22655	16598..17851	+	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
DHB4_RS22665	18423..18764	-	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
DHB4_RS22670	18785..19102	-	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
DHB4_RS25565	19121..19342	-	222	WP_000691994.1	DUF987 domain-containing protein	-
DHB4_RS22675	19351..19827	-	477	WP_000811693.1	RadC family protein	-
DHB4_RS22680	19843..20301	-	459	WP_000211838.1	antirestriction protein	-
DHB4_RS22685	20399..20638	-	240	WP_000194654.1	DUF905 domain-containing protein	-
DHB4_RS22690	20715..21182	-	468	WP_001547765.1	hypothetical protein	-
DHB4_RS26800	21205..21648	-	444	WP_000824223.1	hypothetical protein	-
DHB4_RS26805	21648..21875	-	228	WP_001548158.1	hypothetical protein	-
DHB4_RS26810	21871..22062	-	192	Protein_30	DeoR family transcriptional regulator	-
DHB4_RS22705	22279..23099	-	821	Protein_31	DUF945 domain-containing protein	-
DHB4_RS22710	23191..24054	-	864	WP_001065553.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	yagV/ecpE / yagW/ecpD / yagX/ecpC / yagY/ecpB / yagZ/ecpA / ykgK/ecpR / fdeC	1..232954	232954

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T60556 WP_000854672.1 NZ_CP014271:c18764-18423 [Escherichia coli K-12]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T60556 NZ_CP014271:c18764-18423 [Escherichia coli K-12]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT60556 WP_000070395.1 NZ_CP014271:c19102-18785 [Escherichia coli K-12]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT60556 NZ_CP014271:c19102-18785 [Escherichia coli K-12]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684