60540

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 2813830..2814413 Replicon chromosome
Accession NZ_CP014270
Organism Escherichia coli K-12 strain K-12 DHB4

Toxin (Protein)


Gene name mazF Uniprot ID S1EZP4
Locus tag DHB4_RS14030 Protein ID WP_000254738.1
Coordinates 2813830..2814165 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag DHB4_RS14035 Protein ID WP_000581937.1
Coordinates 2814165..2814413 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DHB4_RS14015 2809717..2811015 - 1299 WP_000036723.1 phosphopyruvate hydratase -
DHB4_RS14020 2811103..2812740 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
DHB4_RS14025 2812968..2813759 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
DHB4_RS14030 2813830..2814165 - 336 WP_000254738.1 endoribonuclease MazF Toxin
DHB4_RS14035 2814165..2814413 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
DHB4_RS14040 2814491..2816470 - 1980 Protein_2689 GTP diphosphokinase -
DHB4_RS14055 2817801..2818061 - 261 Protein_2691 GTP diphosphokinase -
DHB4_RS14060 2818109..2819410 - 1302 WP_000046812.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12098.04 Da        Isoelectric Point: 8.2618

>T60540 WP_000254738.1 NZ_CP014270:c2814165-2813830 [Escherichia coli K-12]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T60540 NZ_CP014270:c2814165-2813830 [Escherichia coli K-12]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT60540 WP_000581937.1 NZ_CP014270:c2814413-2814165 [Escherichia coli K-12]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT60540 NZ_CP014270:c2814413-2814165 [Escherichia coli K-12]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CK9
PDB 5CKH
PDB 5CO7
PDB 3NFC
PDB 5CR2
PDB 5CKB
PDB 5CQY
PDB 5CKD
PDB 5CKE
PDB 5CKF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LMB4

References