Detailed information of TA system TA060540

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 2813830..2814413
• Replicon: chromosome
• Accession: NZ_CP014270
• Organism: Escherichia coli K-12 strain K-12 DHB4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: S1EZP4
• Locus tag: DHB4_RS14030
• Protein ID: WP_000254738.1
• Coordinates: 2813830..2814165 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: S1P3W5
• Locus tag: DHB4_RS14035
• Protein ID: WP_000581937.1
• Coordinates: 2814165..2814413 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DHB4_RS14015	2809717..2811015	-	1299	WP_000036723.1	phosphopyruvate hydratase	-
DHB4_RS14020	2811103..2812740	-	1638	WP_000210878.1	CTP synthase (glutamine hydrolyzing)	-
DHB4_RS14025	2812968..2813759	-	792	WP_001071648.1	nucleoside triphosphate pyrophosphohydrolase	-
DHB4_RS14030	2813830..2814165	-	336	WP_000254738.1	endoribonuclease MazF	Toxin
DHB4_RS14035	2814165..2814413	-	249	WP_000581937.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
DHB4_RS14040	2814491..2816470	-	1980	Protein_2689	GTP diphosphokinase	-
DHB4_RS14055	2817801..2818061	-	261	Protein_2691	GTP diphosphokinase	-
DHB4_RS14060	2818109..2819410	-	1302	WP_000046812.1	23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-109)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12098.04 Da
• Isoelectric Point: 8.2618

>T60540 WP_000254738.1 NZ_CP014270:c2814165-2813830 [Escherichia coli K-12]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Nucleotide

• Length: 336 bp

>T60540 NZ_CP014270:c2814165-2813830 [Escherichia coli K-12]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9355.74 Da
• Isoelectric Point: 4.4665

>AT60540 WP_000581937.1 NZ_CP014270:c2814413-2814165 [Escherichia coli K-12]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Nucleotide

• Length: 249 bp

>AT60540 NZ_CP014270:c2814413-2814165 [Escherichia coli K-12]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• PDB: 1UB4
• PDB: 5CQX
• PDB: 5CK9
• PDB: 5CKH
• PDB: 5CO7
• PDB: 3NFC
• PDB: 5CR2
• PDB: 5CKB
• PDB: 5CQY
• PDB: 5CKD
• PDB: 5CKE
• PDB: 5CKF

Antitoxin

• PDB: 1UB4
• PDB: 5CQX
• PDB: 5CQY
• PDB: 1MVF
• PDB: 2MRN
• PDB: 2MRU
• AlphaFold DB: A0A7U9LMB4