Detailed information of TA system TA060514

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 398751..399369
• Replicon: chromosome
• Accession: NZ_CP014270
• Organism: Escherichia coli K-12 strain K-12 DHB4

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: -
• Locus tag: DHB4_RS01985
• Protein ID: WP_001290581.1
• Coordinates: 398751..398969 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: S1PTH5
• Locus tag: DHB4_RS01990
• Protein ID: WP_000344800.1
• Coordinates: 398995..399369 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DHB4_RS01950	394040..394612	+	573	WP_000779842.1	YbaY family lipoprotein	-
DHB4_RS01955	394643..394954	-	312	WP_000409911.1	MGMT family protein	-
DHB4_RS01965	395333..395686	+	354	WP_000878140.1	DUF1428 domain-containing protein	-
DHB4_RS01970	395728..397278	-	1551	WP_001310610.1	cyclic-guanylate-specific phosphodiesterase PdeB	-
DHB4_RS01975	397442..397912	-	471	WP_000136192.1	YlaC family protein	-
DHB4_RS01980	398028..398579	-	552	WP_000102564.1	maltose O-acetyltransferase	-
DHB4_RS01985	398751..398969	-	219	WP_001290581.1	hemolysin expression modulator Hha	Toxin
DHB4_RS01990	398995..399369	-	375	WP_000344800.1	Hha toxicity modulator TomB	Antitoxin
DHB4_RS01995	399915..403064	-	3150	WP_001132469.1	multidrug efflux RND transporter permease subunit	-
DHB4_RS02000	403087..404280	-	1194	WP_001295324.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8678.06 Da
• Isoelectric Point: 8.9008

>T60514 WP_001290581.1 NZ_CP014270:c398969-398751 [Escherichia coli K-12]
MSEKFLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Nucleotide

• Length: 219 bp

>T60514 NZ_CP014270:c398969-398751 [Escherichia coli K-12]
ATGTCCGAAAAATTTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14557.39 Da
• Isoelectric Point: 4.7395

>AT60514 WP_000344800.1 NZ_CP014270:c399369-398995 [Escherichia coli K-12]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Nucleotide

• Length: 375 bp

>AT60514 NZ_CP014270:c399369-398995 [Escherichia coli K-12]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A7U9QBQ5