Detailed information of TA system TA060489

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 1173690..1174527
• Replicon: chromosome
• Accession: NZ_CP014269
• Organism: Escherichia coli B strain C3029

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: A0A140NB16
• Locus tag: C3029_RS05725
• Protein ID: WP_000227786.1
• Coordinates: 1173690..1174232 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: C3029_RS05730
• Protein ID: WP_001297137.1
• Coordinates: 1174216..1174527 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3029_RS05705	1169220..1170131	-	912	WP_000705853.1	2-dehydropantoate 2-reductase	-
C3029_RS05710	1170299..1170790	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
C3029_RS05715	1170918..1172282	-	1365	WP_001000978.1	MFS transporter	-
C3029_RS05720	1172699..1173634	+	936	WP_001297127.1	sel1 repeat family protein	-
C3029_RS05725	1173690..1174232	-	543	WP_000227786.1	GNAT family N-acetyltransferase	Toxin
C3029_RS05730	1174216..1174527	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
C3029_RS05735	1174712..1175602	-	891	WP_000971336.1	heme o synthase	-
C3029_RS05740	1175614..1175943	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
C3029_RS05745	1175943..1176557	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
C3029_RS05750	1176547..1178538	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
C3029_RS05755	1178560..1179507	-	948	WP_001239440.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19794.98 Da
• Isoelectric Point: 8.3395

>T60489 WP_000227786.1 NZ_CP014269:c1174232-1173690 [Escherichia coli B]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADSAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T60489 NZ_CP014269:c1174232-1173690 [Escherichia coli B]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACTCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT60489 WP_001297137.1 NZ_CP014269:c1174527-1174216 [Escherichia coli B]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT60489 NZ_CP014269:c1174527-1174216 [Escherichia coli B]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A140NB16

Antitoxin

• AlphaFold DB: A0A0E0Y6B3