Detailed information of TA system TA060486

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 771459..772273
• Replicon: chromosome
• Accession: NZ_CP014269
• Organism: Escherichia coli B strain C3029

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: C3029_RS03795
• Protein ID: WP_001054376.1
• Coordinates: 771459..771716 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: C3029_RS03800
• Protein ID: WP_001309181.1
• Coordinates: 771728..772273 (+)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3029_RS03770	766580..767560	+	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
C3029_RS03775	767824..769032	+	1209	WP_001339197.1	IS4-like element ISVsa5 family transposase	-
C3029_RS03780	769481..770721	-	1241	Protein_694	helicase YjhR	-
C3029_RS25325	770837..771082	+	246	Protein_695	GNAT family N-acetyltransferase	-
C3029_RS03795	771459..771716	+	258	WP_001054376.1	hypothetical protein	Toxin
C3029_RS03800	771728..772273	+	546	WP_001309181.1	N-acetyltransferase	Antitoxin
C3029_RS03805	772329..773075	+	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
C3029_RS24710	773244..773462	+	219	Protein_699	hypothetical protein	-
C3029_RS25895	773500..773616	+	117	Protein_700	VOC family protein	-
C3029_RS03810	773861..774982	+	1122	WP_000010829.1	M42 family metallopeptidase	-
C3029_RS03815	774979..775257	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
C3029_RS03820	775269..776582	+	1314	WP_000460843.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	767824..769032	1208

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T60486 WP_001054376.1 NZ_CP014269:771459-771716 [Escherichia coli B]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T60486 NZ_CP014269:771459-771716 [Escherichia coli B]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT60486 WP_001309181.1 NZ_CP014269:771728-772273 [Escherichia coli B]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT60486 NZ_CP014269:771728-772273 [Escherichia coli B]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8