Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3634597..3635251 Replicon chromosome
Accession NZ_CP014268
Organism Escherichia coli B strain C2566

Toxin (Protein)


Gene name cptA Uniprot ID A0A140N853
Locus tag C2566_RS17935 Protein ID WP_000244783.1
Coordinates 3634844..3635251 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag C2566_RS17930 Protein ID WP_000354046.1
Coordinates 3634597..3634863 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2566_RS17905 3629766..3630509 + 744 WP_000951948.1 SDR family oxidoreductase -
C2566_RS17910 3630566..3631999 - 1434 WP_012775976.1 6-phospho-beta-glucosidase BglA -
C2566_RS17915 3632044..3632355 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
C2566_RS17920 3632519..3633178 + 660 WP_000250274.1 hemolysin III family protein -
C2566_RS17925 3633374..3634354 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
C2566_RS17930 3634597..3634863 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
C2566_RS17935 3634844..3635251 + 408 WP_000244783.1 protein YgfX Toxin
C2566_RS17940 3635291..3635812 - 522 WP_001055874.1 flavodoxin FldB -
C2566_RS17945 3635924..3636820 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
C2566_RS17950 3636845..3637555 + 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
C2566_RS17955 3637561..3639294 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15962.85 Da        Isoelectric Point: 11.2669

>T60482 WP_000244783.1 NZ_CP014268:3634844-3635251 [Escherichia coli B]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEISLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T60482 NZ_CP014268:3634844-3635251 [Escherichia coli B]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTAGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT60482 WP_000354046.1 NZ_CP014268:3634597-3634863 [Escherichia coli B]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT60482 NZ_CP014268:3634597-3634863 [Escherichia coli B]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A140N853

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References