Detailed information of TA system TA060463

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 1172356..1173193
• Replicon: chromosome
• Accession: NZ_CP014268
• Organism: Escherichia coli B strain C2566

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: A0A140NB16
• Locus tag: C2566_RS05710
• Protein ID: WP_000227786.1
• Coordinates: 1172356..1172898 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: C2566_RS05715
• Protein ID: WP_001297137.1
• Coordinates: 1172882..1173193 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2566_RS05690	1167886..1168797	-	912	WP_000705853.1	2-dehydropantoate 2-reductase	-
C2566_RS05695	1168965..1169456	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
C2566_RS05700	1169584..1170948	-	1365	WP_001000978.1	MFS transporter	-
C2566_RS05705	1171365..1172300	+	936	WP_001297127.1	sel1 repeat family protein	-
C2566_RS05710	1172356..1172898	-	543	WP_000227786.1	GNAT family N-acetyltransferase	Toxin
C2566_RS05715	1172882..1173193	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
C2566_RS05720	1173378..1174268	-	891	WP_000971336.1	heme o synthase	-
C2566_RS05725	1174280..1174609	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
C2566_RS05730	1174609..1175223	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
C2566_RS05735	1175213..1177204	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
C2566_RS05740	1177226..1178173	-	948	WP_001239440.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19794.98 Da
• Isoelectric Point: 8.3395

>T60463 WP_000227786.1 NZ_CP014268:c1172898-1172356 [Escherichia coli B]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADSAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T60463 NZ_CP014268:c1172898-1172356 [Escherichia coli B]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACTCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT60463 WP_001297137.1 NZ_CP014268:c1173193-1172882 [Escherichia coli B]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT60463 NZ_CP014268:c1173193-1172882 [Escherichia coli B]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A140NB16

Antitoxin

• AlphaFold DB: A0A0E0Y6B3