Detailed information of TA system TA060448

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 44067..44644
• Replicon: plasmid CFSAN023459_02
• Accession: NZ_CP014254
• Organism: Listeria monocytogenes strain CFSAN023459

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A3T2CKN4
• Locus tag: KO07_RS15500
• Protein ID: WP_031644362.1
• Coordinates: 44303..44644 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A3T1XTB0
• Locus tag: KO07_RS15495
• Protein ID: WP_031644363.1
• Coordinates: 44067..44309 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KO07_RS15460	39865..40626	-	762	WP_149027728.1	hypothetical protein	-
KO07_RS15465	40734..41414	-	681	WP_003759224.1	IS6 family transposase	-
KO07_RS15870	41566..41814	-	249	WP_077915060.1	hypothetical protein	-
KO07_RS15475	42222..42605	-	384	WP_071358971.1	DUF3850 domain-containing protein	-
KO07_RS15480	42618..43280	-	663	WP_031645743.1	DUF2786 domain-containing protein	-
KO07_RS15485	43280..43606	-	327	WP_031644500.1	hypothetical protein	-
KO07_RS15490	43770..44006	+	237	WP_031644364.1	hypothetical protein	-
KO07_RS15495	44067..44309	+	243	WP_031644363.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
KO07_RS15500	44303..44644	+	342	WP_031644362.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KO07_RS15505	44673..44858	-	186	WP_031644361.1	hypothetical protein	-
KO07_RS15510	45115..45504	+	390	WP_003759346.1	hypothetical protein	-
KO07_RS15515	45488..45721	+	234	WP_072235491.1	hypothetical protein	-
KO07_RS15520	45722..46222	+	501	WP_060868968.1	hypothetical protein	-
KO07_RS15525	46586..47575	-	990	WP_003759341.1	hypothetical protein	-
KO07_RS15530	47582..47842	-	261	WP_003759339.1	helix-turn-helix transcriptional regulator	-
KO07_RS15535	47952..48497	-	546	WP_139028137.1	hypothetical protein	-
KO07_RS15540	48727..49104	-	378	WP_003759336.1	hypothetical protein	-
KO07_RS15545	49123..49618	-	496	Protein_60	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..52687	52687

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-84)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 14629.86 Da
• Isoelectric Point: 12.8531

>T60448 WP_031644362.1 NZ_CP014254:44303-44644 [Listeria monocytogenes]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 342 bp

>T60448 NZ_CP014254:44303-44644 [Listeria monocytogenes]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 10400.12 Da
• Isoelectric Point: 12.7030

>AT60448 WP_031644363.1 NZ_CP014254:44067-44309 [Listeria monocytogenes]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
K

Nucleotide

• Length: 243 bp

>AT60448 NZ_CP014254:44067-44309 [Listeria monocytogenes]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAA

Structures

Toxin

• AlphaFold DB: A0A3T2CKN4

Antitoxin

• AlphaFold DB: A0A3T1XTB0