Detailed information of TA system TA060427

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 2638025..2638608
• Replicon: chromosome
• Accession: NZ_CP014230
• Organism: Desulfomicrobium orale DSM 12838

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: AXF15_RS13780
• Protein ID: WP_083518041.1
• Coordinates: 2638354..2638608 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A0X8JRZ0
• Locus tag: AXF15_RS12395
• Protein ID: WP_066608091.1
• Coordinates: 2638025..2638357 (-)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AXF15_RS12365	2633235..2634359	+	1125	WP_211258988.1	P-type conjugative transfer protein TrbL	-
AXF15_RS12370	2634362..2635060	+	699	WP_083518040.1	conjugal transfer protein	-
AXF15_RS12375	2635065..2635973	+	909	WP_066608081.1	P-type conjugative transfer protein TrbG	-
AXF15_RS12380	2635973..2636395	+	423	WP_066608083.1	hypothetical protein	-
AXF15_RS12385	2636392..2637555	+	1164	WP_066608085.1	hypothetical protein	-
AXF15_RS12390	2637644..2637982	-	339	WP_066608088.1	type II toxin-antitoxin system HicB family antitoxin	-
AXF15_RS12395	2638025..2638357	-	333	WP_066608091.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
AXF15_RS13780	2638354..2638608	-	255	WP_083518041.1	type II toxin-antitoxin system HicA family toxin	Toxin
AXF15_RS12400	2638656..2640572	+	1917	WP_066608093.1	type IV secretory system conjugative DNA transfer family protein	-
AXF15_RS12405	2640577..2641131	+	555	WP_066608096.1	conjugative transfer signal peptidase TraF	-
AXF15_RS12410	2641239..2643320	+	2082	WP_066608099.1	DUF1738 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-81)

Antitoxin

• Uncharacterised protein family HicB (54-103)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9244.55 Da
• Isoelectric Point: 10.7342

>T60427 WP_083518041.1 NZ_CP014230:c2638608-2638354 [Desulfomicrobium orale DSM 12838]
MNSKQRKTLEAIFADPIRSGIPWADIESLFVALGAEITEGRGSRVRISLNGVLATFHRPHPERNTDKGAVKSVRRFLETA
GGEI

Nucleotide

• Length: 255 bp

>T60427 NZ_CP014230:c2638608-2638354 [Desulfomicrobium orale DSM 12838]
ATGAACAGCAAGCAGCGGAAGACGCTGGAAGCCATTTTTGCCGACCCGATTCGATCCGGTATCCCATGGGCGGATATTGA
AAGCCTTTTCGTAGCCCTTGGCGCTGAAATCACGGAAGGAAGAGGCTCTCGGGTACGGATCAGCCTTAATGGCGTGCTTG
CTACGTTCCACAGGCCGCACCCGGAAAGAAACACAGATAAGGGAGCCGTGAAATCGGTCAGGCGTTTTCTGGAAACTGCG
GGAGGAGAAATATGA

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 12483.92 Da
• Isoelectric Point: 4.7351

>AT60427 WP_066608091.1 NZ_CP014230:c2638357-2638025 [Desulfomicrobium orale DSM 12838]
MNCLNYRGYTGCFEYDSEADIFHGEVLDLNDVITFQGRSIDELKAALKDSVEDYLEFCTTEGKTPEKPYSGRFNLRIAPD
LHRRAAAEAGKNRKSLNSWVADVIRDAVYP

Nucleotide

• Length: 333 bp

>AT60427 NZ_CP014230:c2638357-2638025 [Desulfomicrobium orale DSM 12838]
ATGAATTGCCTGAATTACAGAGGCTATACCGGATGCTTTGAGTATGATTCCGAAGCGGATATTTTTCATGGAGAAGTTCT
CGACCTGAACGATGTCATTACGTTTCAGGGCCGCTCCATTGATGAGCTTAAAGCCGCTCTGAAAGATTCCGTGGAAGATT
ACCTTGAGTTCTGCACCACAGAAGGAAAAACCCCGGAAAAGCCTTATTCCGGACGTTTCAACCTGCGTATTGCCCCTGAT
CTGCATCGGCGGGCAGCTGCTGAGGCGGGAAAAAACCGCAAGAGCCTCAACAGCTGGGTCGCCGATGTCATCAGAGATGC
CGTCTACCCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0X8JRZ0