Detailed information of TA system TA060426

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 2330786..2331377
• Replicon: chromosome
• Accession: NZ_CP014230
• Organism: Desulfomicrobium orale DSM 12838

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: AXF15_RS13670
• Protein ID: WP_083518010.1
• Coordinates: 2331060..2331377 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A120KNA6
• Locus tag: AXF15_RS10890
• Protein ID: WP_066607316.1
• Coordinates: 2330786..2331076 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AXF15_RS10865	2326198..2326584	-	387	WP_066607305.1	hypothetical protein	-
AXF15_RS14085	2326902..2327303	+	402	WP_151192360.1	hypothetical protein	-
AXF15_RS10870	2327306..2328031	+	726	WP_066607308.1	hypothetical protein	-
AXF15_RS10875	2328033..2328461	+	429	WP_066607311.1	hypothetical protein	-
AXF15_RS13660	2328478..2328762	-	285	WP_083518008.1	type II toxin-antitoxin system RelE/ParE family toxin	-
AXF15_RS10880	2328759..2328992	-	234	WP_211258979.1	CopG family ribbon-helix-helix protein	-
AXF15_RS13665	2329081..2330295	-	1215	WP_083518009.1	Fic family protein	-
AXF15_RS10890	2330786..2331076	-	291	WP_066607316.1	putative addiction module antidote protein	Antitoxin
AXF15_RS13670	2331060..2331377	-	318	WP_083518010.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AXF15_RS14090	2331574..2332182	-	609	WP_151192361.1	hypothetical protein	-
AXF15_RS10895	2332207..2332563	-	357	WP_083518011.1	helix-turn-helix domain-containing protein	-
AXF15_RS10900	2332682..2332918	+	237	WP_151192362.1	hypothetical protein	-
AXF15_RS14095	2332903..2333121	+	219	WP_151192363.1	hypothetical protein	-
AXF15_RS10905	2333130..2333417	+	288	WP_066607323.1	hypothetical protein	-
AXF15_RS10910	2333647..2333856	+	210	WP_066607326.1	hypothetical protein	-
AXF15_RS14100	2334006..2335733	+	1728	WP_151192364.1	hypothetical protein	-
AXF15_RS14105	2335736..2336086	+	351	WP_151192365.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-96)

Antitoxin

• Uncharacterized protein HI1420 (3-90)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12315.00 Da
• Isoelectric Point: 9.8167

>T60426 WP_083518010.1 NZ_CP014230:c2331377-2331060 [Desulfomicrobium orale DSM 12838]
MNTFYQTDQFSNWLQKLRDIQAKARIVARIKSAEVGNLGDCAPVGEGVSEMRLHFGPGYRLYFWKKETRVYWLLIGGNKS
SQQKDIRRAQALRREIEEIEHGERE

Nucleotide

• Length: 318 bp

>T60426 NZ_CP014230:c2331377-2331060 [Desulfomicrobium orale DSM 12838]
ATGAATACCTTCTACCAAACAGACCAGTTTTCAAATTGGCTACAAAAACTCCGAGATATACAAGCCAAGGCACGAATAGT
TGCCAGAATCAAGTCGGCGGAAGTTGGGAATTTAGGAGACTGCGCACCTGTTGGAGAAGGCGTTTCAGAAATGCGGTTAC
ATTTTGGACCGGGATACCGCTTGTATTTCTGGAAAAAAGAAACTCGGGTTTACTGGTTACTCATTGGAGGGAACAAAAGT
TCCCAGCAAAAAGATATCCGGCGCGCCCAAGCACTTCGGCGTGAAATCGAGGAGATAGAACATGGTGAACGTGAGTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10400.05 Da
• Isoelectric Point: 5.0651

>AT60426 WP_066607316.1 NZ_CP014230:c2331076-2330786 [Desulfomicrobium orale DSM 12838]
MVNVSRFDAVQYLDSEEMMAAYLEAAMEDQNPDVFLAALADVAKARGIAQLAKDSGLGRESLYKSLAPGAKPRFETIMKI
TKSLGIPLRIQAPGQV

Nucleotide

• Length: 291 bp

>AT60426 NZ_CP014230:c2331076-2330786 [Desulfomicrobium orale DSM 12838]
ATGGTGAACGTGAGTAGATTCGACGCAGTACAGTATCTGGACAGCGAAGAAATGATGGCGGCCTATCTGGAAGCAGCGAT
GGAGGATCAGAACCCTGATGTATTTCTGGCCGCCCTTGCTGATGTGGCCAAGGCGCGAGGTATCGCACAGCTTGCAAAAG
ATAGTGGCTTGGGACGGGAAAGCCTCTACAAGAGCCTAGCGCCCGGAGCAAAGCCGCGATTTGAAACAATCATGAAAATC
ACAAAAAGCCTAGGAATCCCTTTACGCATACAAGCGCCTGGGCAAGTATGA

Structures

Antitoxin

• AlphaFold DB: A0A120KNA6