Detailed information of TA system TA060412

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 4348210..4348848
• Replicon: chromosome
• Accession: NZ_CP014225
• Organism: Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: AW869_RS21535
• Protein ID: WP_000813794.1
• Coordinates: 4348210..4348386 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: AW869_RS21540
• Protein ID: WP_001270286.1
• Coordinates: 4348432..4348848 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AW869_RS21515	4343829..4345043	-	1215	WP_001326689.1	BenE family transporter YdcO	-
AW869_RS21520	4345096..4345632	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
AW869_RS21525	4345705..4347666	+	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
AW869_RS21530	4347758..4347988	-	231	WP_000494244.1	YncJ family protein	-
AW869_RS21535	4348210..4348386	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
AW869_RS21540	4348432..4348848	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
AW869_RS21545	4348927..4350333	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
AW869_RS21550	4350578..4351723	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
AW869_RS21555	4351741..4352754	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
AW869_RS21560	4352755..4353696	+	942	WP_001251304.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T60412 WP_000813794.1 NZ_CP014225:4348210-4348386 [Escherichia coli str. K-12 substr. MG1655]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T60412 NZ_CP014225:4348210-4348386 [Escherichia coli str. K-12 substr. MG1655]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT60412 WP_001270286.1 NZ_CP014225:4348432-4348848 [Escherichia coli str. K-12 substr. MG1655]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT60412 NZ_CP014225:4348432-4348848 [Escherichia coli str. K-12 substr. MG1655]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB