Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3318831..3319449 Replicon chromosome
Accession NZ_CP014225
Organism Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AW869_RS16330 Protein ID WP_001291435.1
Coordinates 3318831..3319049 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AW869_RS16335 Protein ID WP_000344800.1
Coordinates 3319075..3319449 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AW869_RS16295 3314120..3314692 + 573 WP_000779842.1 YbaY family lipoprotein -
AW869_RS16300 3314723..3315034 - 312 WP_000409911.1 MGMT family protein -
AW869_RS16310 3315413..3315766 + 354 WP_000878140.1 DUF1428 domain-containing protein -
AW869_RS16315 3315808..3317358 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AW869_RS16320 3317522..3317992 - 471 WP_000136192.1 YlaC family protein -
AW869_RS16325 3318108..3318659 - 552 WP_000102564.1 maltose O-acetyltransferase -
AW869_RS16330 3318831..3319049 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
AW869_RS16335 3319075..3319449 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AW869_RS16340 3319995..3323144 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
AW869_RS16345 3323167..3324360 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T60401 WP_001291435.1 NZ_CP014225:c3319049-3318831 [Escherichia coli str. K-12 substr. MG1655]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T60401 NZ_CP014225:c3319049-3318831 [Escherichia coli str. K-12 substr. MG1655]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT60401 WP_000344800.1 NZ_CP014225:c3319449-3319075 [Escherichia coli str. K-12 substr. MG1655]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT60401 NZ_CP014225:c3319449-3319075 [Escherichia coli str. K-12 substr. MG1655]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References