Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yafW-ykfI/CbtA-CbeA |
| Location | 3102069..3102748 | Replicon | chromosome |
| Accession | NZ_CP014225 | ||
| Organism | Escherichia coli str. K-12 substr. MG1655 strain K-12 | ||
Toxin (Protein)
| Gene name | ykfI | Uniprot ID | P77692 |
| Locus tag | AW869_RS15215 | Protein ID | WP_000854672.1 |
| Coordinates | 3102069..3102410 (-) | Length | 114 a.a. |
Antitoxin (Protein)
| Gene name | yafW | Uniprot ID | Q47684 |
| Locus tag | AW869_RS15220 | Protein ID | WP_000070395.1 |
| Coordinates | 3102431..3102748 (-) | Length | 106 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| AW869_RS15190 | 3097418..3097747 | + | 330 | Protein_2873 | sigma factor-binding protein Crl | - |
| AW869_RS15195 | 3097786..3098841 | - | 1056 | WP_000749863.1 | phosphoporin PhoE | - |
| AW869_RS15200 | 3099129..3100232 | + | 1104 | WP_001285288.1 | glutamate 5-kinase | - |
| AW869_RS15205 | 3100244..3101497 | + | 1254 | WP_000893278.1 | glutamate-5-semialdehyde dehydrogenase | - |
| AW869_RS15215 | 3102069..3102410 | - | 342 | WP_000854672.1 | type IV toxin-antitoxin system toxin YkfI | Toxin |
| AW869_RS15220 | 3102431..3102748 | - | 318 | WP_000070395.1 | type IV toxin-antitoxin system antitoxin YafW | Antitoxin |
| AW869_RS24235 | 3102767..3102988 | - | 222 | WP_000691994.1 | DUF987 domain-containing protein | - |
| AW869_RS15225 | 3102997..3103473 | - | 477 | WP_000811693.1 | RadC family protein | - |
| AW869_RS15230 | 3103489..3103947 | - | 459 | WP_000211838.1 | antirestriction protein | - |
| AW869_RS15235 | 3104045..3104284 | - | 240 | WP_000194654.1 | DUF905 domain-containing protein | - |
| AW869_RS15240 | 3104361..3104828 | - | 468 | WP_001547765.1 | hypothetical protein | - |
| AW869_RS25695 | 3104851..3105294 | - | 444 | WP_000824223.1 | hypothetical protein | - |
| AW869_RS25700 | 3105294..3105521 | - | 228 | WP_001548158.1 | hypothetical protein | - |
| AW869_RS25705 | 3105517..3105708 | - | 192 | Protein_2886 | DeoR family transcriptional regulator | - |
| AW869_RS15255 | 3105925..3106746 | - | 822 | WP_000197389.1 | DUF945 domain-containing protein | - |
| AW869_RS15260 | 3106838..3107701 | - | 864 | WP_001065553.1 | GTPase family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Prophage | - | - | 3095268..3135762 | 40494 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 114 a.a. Molecular weight: 12905.01 Da Isoelectric Point: 9.6543
>T60400 WP_000854672.1 NZ_CP014225:c3102410-3102069 [Escherichia coli str. K-12 substr. MG1655]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR
Download Length: 342 bp
>T60400 NZ_CP014225:c3102410-3102069 [Escherichia coli str. K-12 substr. MG1655]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA
Antitoxin
Download Length: 106 a.a. Molecular weight: 11939.59 Da Isoelectric Point: 6.7391
>AT60400 WP_000070395.1 NZ_CP014225:c3102748-3102431 [Escherichia coli str. K-12 substr. MG1655]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR
Download Length: 318 bp
>AT60400 NZ_CP014225:c3102748-3102431 [Escherichia coli str. K-12 substr. MG1655]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|