Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 2645536..2646131 Replicon chromosome
Accession NZ_CP014225
Organism Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)


Gene name chpB Uniprot ID U9XNP6
Locus tag AW869_RS12990 Protein ID WP_000239577.1
Coordinates 2645781..2646131 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag AW869_RS12985 Protein ID WP_001223208.1
Coordinates 2645536..2645787 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AW869_RS12975 2641201..2644980 + 3780 WP_000060911.1 autotransporter assembly complex protein TamB -
AW869_RS12980 2644983..2645324 + 342 WP_001219160.1 gamma-glutamylcyclotransferase -
AW869_RS12985 2645536..2645787 + 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
AW869_RS12990 2645781..2646131 + 351 WP_000239577.1 endoribonuclease toxin ChpB Toxin
AW869_RS12995 2646211..2646741 - 531 WP_000055075.1 inorganic diphosphatase -
AW869_RS13000 2647051..2648007 + 957 WP_000265913.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
AW869_RS13005 2648147..2649649 + 1503 WP_000205805.1 sugar ABC transporter ATP-binding protein -
AW869_RS13010 2649663..2650685 + 1023 WP_001313531.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12492.40 Da        Isoelectric Point: 5.5572

>T60392 WP_000239577.1 NZ_CP014225:2645781-2646131 [Escherichia coli str. K-12 substr. MG1655]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLHARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T60392 NZ_CP014225:2645781-2646131 [Escherichia coli str. K-12 substr. MG1655]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT60392 WP_001223208.1 NZ_CP014225:2645536-2645787 [Escherichia coli str. K-12 substr. MG1655]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT60392 NZ_CP014225:2645536-2645787 [Escherichia coli str. K-12 substr. MG1655]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XNP6

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References