Detailed information of TA system TA060365

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2726596..2727247
• Replicon: chromosome
• Accession: NZ_CP014215
• Organism: Acinetobacter baumannii strain YU-R612

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S3TWT7
• Locus tag: AXA63_RS13220
• Protein ID: WP_000838146.1
• Coordinates: 2727065..2727247 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A0J1AAF8
• Locus tag: AXA63_RS13215
• Protein ID: WP_000966689.1
• Coordinates: 2726596..2727000 (-)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AXA63_RS13200	2721629..2721970	-	342	WP_001281459.1	phage tail protein	-
AXA63_RS13205	2722088..2726053	-	3966	WP_000191304.1	tape measure protein	-
AXA63_RS13210	2726114..2726515	-	402	WP_000720591.1	hypothetical protein	-
AXA63_RS13215	2726596..2727000	-	405	WP_000966689.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
AXA63_RS13220	2727065..2727247	-	183	WP_000838146.1	type II toxin-antitoxin system HicA family toxin	Toxin
AXA63_RS21090	2727297..2727464	-	168	WP_171254609.1	hypothetical protein	-
AXA63_RS13225	2727578..2728111	-	534	WP_001185622.1	hypothetical protein	-
AXA63_RS13230	2728156..2729085	-	930	WP_000094300.1	hypothetical protein	-
AXA63_RS13235	2729193..2729405	-	213	WP_000121166.1	hypothetical protein	-
AXA63_RS13240	2729407..2729805	-	399	WP_001132270.1	hypothetical protein	-
AXA63_RS13245	2729807..2730175	-	369	WP_000539752.1	hypothetical protein	-
AXA63_RS13250	2730147..2730551	-	405	WP_000248324.1	hypothetical protein	-
AXA63_RS13255	2730560..2730928	-	369	WP_000524231.1	hypothetical protein	-
AXA63_RS13260	2730930..2731319	-	390	WP_000008492.1	hypothetical protein	-
AXA63_RS13265	2731324..2731989	-	666	WP_000767881.1	stress-responsive nuclear envelope protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2703025..2757639	54614

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-126)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6695.84 Da
• Isoelectric Point: 10.5523

>T60365 WP_000838146.1 NZ_CP014215:c2727247-2727065 [Acinetobacter baumannii]
VKSLDLIKMIEADGWYEVRVSGSHHHFKHPTKKGLVTIPHPKKDLPNGTVKSILKQAGLN

Nucleotide

• Length: 183 bp

>T60365 NZ_CP014215:c2727247-2727065 [Acinetobacter baumannii]
GTGAAAAGTCTGGATTTAATCAAAATGATTGAAGCAGACGGTTGGTATGAGGTTAGGGTTTCAGGAAGTCATCATCACTT
CAAACACCCAACCAAAAAGGGATTAGTAACAATCCCTCATCCTAAAAAGGATTTACCAAACGGAACTGTTAAAAGCATTT
TGAAGCAAGCGGGTCTAAATTGA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14769.78 Da
• Isoelectric Point: 4.5000

>AT60365 WP_000966689.1 NZ_CP014215:c2727000-2726596 [Acinetobacter baumannii]
MLYPIAIERGTDTEAFGVSVPDIPGCFSAGDTLYEAIENVKEAISGHLEILAEDGEEIPLASDVSKFIDQEDYRGMIWAV
TEVDVSRYLGKPEKINVTLPSRLIRKIDDNVGKDKRFKTRSAFLAAGAEKLLHA

Nucleotide

• Length: 405 bp

>AT60365 NZ_CP014215:c2727000-2726596 [Acinetobacter baumannii]
ATGTTATATCCAATTGCTATTGAAAGAGGTACAGACACCGAAGCCTTTGGTGTCTCCGTTCCAGATATTCCAGGGTGTTT
CAGCGCAGGCGATACATTATATGAAGCTATCGAGAATGTTAAAGAGGCTATTTCTGGCCACTTGGAAATCCTAGCAGAAG
ATGGAGAGGAGATTCCTTTAGCATCTGATGTCAGTAAGTTTATTGACCAAGAAGATTATAGAGGTATGATCTGGGCAGTT
ACTGAAGTTGATGTTAGCCGTTACTTAGGTAAACCAGAAAAAATCAATGTTACTTTACCAAGCCGATTAATTCGGAAGAT
TGATGATAATGTTGGTAAAGATAAAAGATTTAAAACTCGCTCTGCTTTTTTGGCCGCTGGTGCTGAAAAGCTACTACATG
CTTAA

Structures

Toxin

• AlphaFold DB: S3TWT7

Antitoxin

• AlphaFold DB: A0A0J1AAF8